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通过meQTL映射对牛精子DNA甲基化的基因调节

Corentin Fouéré^1,2, Valentin Costes^3,4,5, Chris Hozé^3,6

¹Eliance, 149 Rue de Bercy, Paris, 75012, France. corentin.fouere@inrae.fr.

|August 23, 2025

概括

这项研究确定了牛精中的甲基化QTLs (meQTLs),揭示了精子DNA甲基化的遗传调节. 这些发现增强了对父亲遗传的表观遗传标记及其在表现特征中的潜在作用的理解.

科学领域:

表观遗传学
基因组学
动物养殖

背景情况:

基因甲基化 (DNAm) 在功能上很重要,并受到基因变异 (meQTL) 的影响.
大多数meQTL研究都集中在人类血液上,因此精子DNAm调节在很大程度上未被探索.
牛精子DNAm调节对于理解父性遗传标记至关重要.

研究的目的:

探索牛精子中DNA甲基化的遗传调节.
在精子中识别 cis 和 trans 甲基化 QTL (meQTL).
了解精子meQTL的遗传性和基因组分布.

主要方法:

在405头霍尔斯坦公牛的精子中进行全基因组关联研究 (GWAS).
减少表示二硫酸盐测序 (RRBS) 用于DNAm测量.
对cis-meQTLs,遗传性和基因组注释的分析.

主要成果:

DNAm的遗传率估计为0.26,其中76%高于0.1.
在32. 9%的CpG中发现了cis- meQTL,在3. 6%的CpG中发现了trans- meQTL,在1. 0%的CpG中发现了trans- meQTL.
发现了8个涉及表观遗传调节的跨meQTL热点,但在血液mDNA中没有观察到类似的效果.

结论:

在牛精子中首次检测和描述meQTLs.
改善了对父性遗传DNAm传播的理解.
为预测动物表现特征提供表观遗传见解.

关键词:

牛类动物 DNA 甲基化在GWAS 在 MeQTL 映射精子

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