乳酸细菌发酵的大豆蛋白凝中的调节11S/7S比:对物理化学特性和过敏性的影响
Xiaocui Lu1, Linjing Yao1, Jie He2
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Journal of the science of food and agriculture
|August 23, 2025
概括
甘氨酸 (11S) 与β- 甘氨酸 (7S) 的比例显著影响凝形成和过敏性. 超过1.0的比率加快凝并降低过敏性,特别是在pH值6.0时.
科学领域:
- 食品科学
- 蛋白质化学
- 过敏性研究
背景情况:
- 乳酸细菌发酵导致大豆蛋白凝固,影响凝和过敏性.
- 像β-凝聚素 (7S) 和甘氨酸 (11S) 这样的大豆蛋白具有不同的凝和过敏性质.
- 不同的11S/7S比对大豆蛋白凝动力学和过敏性的影响尚不清楚.
研究的目的:
- 研究控制的11S/7S比对大豆蛋白凝和聚合的影响.
- 确定不同的11S/7S比如何影响发酵过程中大豆蛋白的过敏性.
- 阐明11S/7S比率和pH对大豆蛋白聚合和免疫活性的协同作用.
主要方法:
- 在受控的pH梯度下对有变化的11S/7S比率的大豆蛋白系统进行系统的研究.
- 对凝形成动力学,度和颗粒大小的分析.
- 凝矩阵的显微检查和电泳性移动性研究.
- 抗原性和免疫球蛋白E结合能力的评估.
主要成果:
- 较高的11S球蛋白含量加速了蛋白质凝,其凝率>1.
- 11S/7S比> 1.0促进了蛋白质聚合,导致更密集的凝矩阵.
- 在pH值6. 0下,11S/7S比>1. 0导致抗原性降低,IgE结合率降低.
结论:
- 11S/7S比对控制大豆蛋白凝性质,加速形成和增强聚合至关重要.
- 超过1.0的11S/7S比提高了大豆蛋白质基质密度,并显著降低了过敏性.
- 在pH值为6.0且11S/7S比超过1.0时,可以达到最佳的抗原性降低.
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