相关实验视频

Updated: Sep 10, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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使用远距离位置预测和智能图书馆设计,将烟草蚀刻病毒蛋白酶设计为无痕裂变平台

Martijn P Bemelmans1, Bach-Ngan Wetzel2, Florian G Neusius2

  • 1SynBiofoundry@TUM, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany.

ACS synthetic biology
|August 23, 2025
PubMed
概括

研究人员设计了一种新的烟草蚀刻病毒蛋白酶 (TEVp) 变体,TEVp-C1,以实现无痕蛋白标签的去除. 这种改善的蛋白酶扩大了基质的特异性,允许在更多的氨基酸之后进行切割以获取本地.

关键词:
在 TEV 蛋白酶偏离部位的突变发生图形网络分析蛋白质工程没有痕迹的裂

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Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

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相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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