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Updated: May 3, 2026

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使用刺激性排放耗尽显微镜对酵母过氧体膜蛋白及其结合伙伴进行局部化

Frank N Mol1, Eline M F de Lange2, Ida J van der Klei3

  • 1Molecular Biophysics, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG Groningen, the Netherlands.

STAR protocols
|August 23, 2025
PubMed
概括

这项研究详细介绍了使用刺激排放耗尽 (STED) 纳米镜检测汉氏菌多态体内多氧体膜蛋白的新方案. 它可以检查Pex3和Atg30等关键蛋白之间的动态同位素.

关键词:
细胞生物学基于细胞的测试显微镜

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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