吸收的积在线粒体中
Mirna Rita Tenan1, Stefano Jacopo Mandriota1, André-Pascal Sappino1
1Laboratoire de Cancérogenèse Environnementale, Fondation des Grangettes, Chêne-Bougeries 1224, Switzerland.
概括
这项研究表明,每天吸收的有毒会积聚在哺乳动物的线粒体内,而不是内网或内体. 这一发现澄清了摄入的亚细胞位置.
科学领域:
- 毒理学
- 细胞生物学
- 生物化学
背景情况:
- 是一种有毒的元素,被怀疑是致癌物,每天从各种产品中广泛吸收.
- 吸收的通过全身循环,主要通过转移素 (TF),并积聚在人体器官中.
- 内部化的精确亚细胞定位仍不清楚,尽管此前认为它会在核附近的颗粒状-网状有机体中积聚.
研究的目的:
- 在细胞吸收后识别积聚的特定亚细胞器官.
- 调查内网 (ER) 或内体是否是积的主要部位.
- 确定负责封存的确切细胞区.
主要方法:
- 开发了一种将Lumogallion染色 (用于检测) 与器官特异性免疫光结合在一起的协议.
- 使用的MCF10A人类乳腺上皮细胞暴露于化物 (AlCl3).
- 将局部化与内分泌网膜 (Calreticulin) 和转移素受体1 (TFR1) 的标记物进行比较,并与线粒体探针MitoTracker共同局部化.
主要成果:
- 与卡尔雷蒂库林没有共同局部化,这表明ER不是主要的积累点.
- 与转移素受体1 (TFR1) 没有共同定位,这表明内分泌物不太可能参与其内化.
- 在人类和小鼠乳腺上皮细胞中,特异性Lumogallion光与MitoTracker光紧密地共定位,特别是在周核区域.
结论:
- 细胞内并不是细胞内的主要积聚点.
- 强有力的实验证据表明,在细胞吸收时会在哺乳动物的线粒体中积聚.
- 这项研究澄清了内化的亚细胞局部,确定线粒体为关键细胞器.
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