来自五种食用真菌的β-葡萄糖的结构特征和Dectin-1/Toll类受体激活的结构-活性关系
Liping Liu1, Jie Feng1, Zerou Lian1
1Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
Food chemistry
|August 23, 2025
概括
可食用真菌的β- 葡萄糖具有增强免疫力的作用. 它们的特定分支结构增强了与模式识别受体 (PRR) 的相互作用,提供了加强免疫力的新途径.
科学领域:
- 免疫学
- 菌群学
- 生物化学
背景情况:
- 天然β- 葡萄糖具有免疫调节特性.
- 这些效应由模式识别受体 (PRR) 介导.
- 了解β-葡萄糖的结构-活性关系对于利用它们的健康益处至关重要.
研究的目的:
- 研究可食真菌中的β-葡萄糖的结构特征.
- 分析它们与关键模式识别受体 (PRR) 的结合相互作用.
- 确定β-葡萄糖结构与免疫刺激活性之间的关系.
主要方法:
- 来自食用真菌的五种高分子量β-葡萄糖分量的表征.
- 分析分支的比例和结构特征.
- 表面等离子体共振和细胞测定以评估受体结合和激活.
- 分子对接模拟以阐明结合机制.
主要成果:
- 已识别出具有O-6分支和分支比为1:2至1:9的β-→3) -D-葡萄糖.
- 对于Dectin-1,TLR4和TLR2受体具有明显的结合和激活能力.
- 显示出免疫刺激活性对分支比的显著依赖性,其中HEP20 (1:3) 和PEP20 (1:4) 是高度活跃的.
- 分子对接显示了通过键在受体单体/二元体内HEP20/PEP20重复单元的最佳结合.
结论:
- 阐明了免疫受体和β- 1→3,1→6) - 葡萄糖之间的相互作用机制,分支模式不同.
- 突出了分支比在确定β- 葡萄糖有效性的关键作用.
- 建议使用特定结构的β-葡萄糖进行免疫增强的新方法.
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