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从青春期前和成年绵羊中分离的积卵细胞的转录形状

Nicola Antonio Martino¹, Letizia Temerario¹, Francesco Parisi¹

¹Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, Valenzano, Italy.

|August 23, 2025

概括

这项研究揭示了青春期前和成年绵羊之间的基因表达细胞 (CCs) 的关键差异,确定了卵细胞质量的潜在非侵入性生物标志物. 这些发现提高了对生殖衰老和卵细胞发育的理解.

科学领域:

生殖生物学
基因组学
动物科学

背景情况:

累积细胞 (CCs) 对于卵细胞的发育和受精至关重要.
在CC中基因表达分析提供了识别卵细胞质量的非侵入性生物标志物的途径.

研究的目的:

为了比较在实验室中成熟的青春期和成年羊卵细胞中的CCs的转录形状.
确定CC基因表达的年龄相关差异及其功能影响.

主要方法:

在实验室中成熟了来自青春期前 (< 6 个月) 和成年 (3 - 5 年) 捐赠者的羊卵细胞复合体 (COC).
从成熟的卵细胞进行了深度转录组测序.
进行了差异基因表达分析 (DESeq2) 和功能丰富分析 (GO,KEGG).

主要成果:

在青春期前和成年羊中分别发现了1024个不同表达的基因 (DEGs) (819个上调,205个下调).
确定了参与芳酶活性,生长因子结合,线粒体功能和细胞-细胞/矩阵相互作用的关键基因.
特定的基因如CYP19A,ERBB3,IGFBP2/3,SOD1以及各种整体蛋白,原蛋白和原蛋白显示出与年龄相关的显著表达变化.

结论:

与捐赠者的年龄相关的CC存在显著的分子差异.
这些与年龄相关的基因表达模式提供了对卵细胞发育和质量的洞察.
已识别的DEG代表了评估成年羊卵细胞质量的潜在非侵入性生物标志物.

关键词:

成年羊累积细胞非侵入性生物标志物卵细胞质量在青春期前的羔羊转录组分析

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