在葡萄中促进果的CPPU反应中,VlMYB12和VlDOF1.5共同向VlCKX6促进剂
Qiaofang Shi1, Yiting Liu1, Ruxin Wang1
1College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China; Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang, 471023, Henan, China.
Plant physiology and biochemistry : PPB
|August 23, 2025
概括
像CPPU这样的植物生长调节剂通过改变细胞激素水平来改善葡萄水果. 研究人员确定了由VlMYB12和VlDOF1.5调节的VlCKX6基因是提高果实产量的关键.
科学领域:
- 植物生物学
- 农业科学
- 分子遗传学
背景情况:
- 葡萄 (Vitis vinifera L.) 的产量和质量受到年轻果群的限制.
- 合成细胞因子CPPU增强葡萄果,但其机制尚不清楚.
- CPPU会影响葡萄中的内源性细胞因素水平和VlCKX6基因表达.
研究的目的:
- 阐明VlCKX6在调节葡萄果中的分子机制.
- 分析VlCKX6基因对CPPU治疗的功能和调节.
主要方法:
- 在CPPU处理的葡萄果中进行基因表达分析 (VlCKX6).
- 在各种治疗 (MeJA,CPPU,ABA) 过程中,进行GUS活性测定以确认VlCKX6促进剂活性.
- 在烟草中过度表达VlCKX6,以观察表型效应.
- 酵母单杂交和双化酶试验以确定VlCKX6促进剂结合因子 (VlMYB12,VlDOF1.5).
主要成果:
- 在年轻的葡萄果实,花朵和枝条中,CPPU显著上调了VlCKX6的表达.
- MeJA和CPPU增强了VlCKX6促进剂的活性,而ABA则抑制了它.
- 烟草中VlCKX6的过度表达导致了细胞因子缺乏的表型.
- 证实VlMYB12和VlDOF1.5直接与VlCKX6促进体结合.
结论:
- 通过调节细胞激素水平,VlCKX6在调节葡萄水果中的作用至关重要.
- VlMYB12和VlDOF1.5直接调节VlCKX6的表达,影响细胞激素水平和果.
- 这项研究为了解CPPU的机制和开发高产葡萄品种提供了理论基础.
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