水溶性维生素的样品制备和分析方法的进步和挑战:在食品,临床和制药样品中的应用
Tikeshwari1, Kamlesh Shrivas2, Khushali Tandey2
1School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010, India; Chandrapal Dadsena Govt. College, Pithora, Mahasamund, Chhattisgarh 493551, India.
精确分析水溶性维生素 (WSV),如维生素C和维生素B,对于健康至关重要. 本审查涵盖了先进的样本制备和分析方法,包括纳米材料,以改善各种样本的WSV确定.
科学领域:
- 分析化学
- 营养科学
- 临床化学
背景情况:
- 水溶性维生素 (WSV) 是许多生理功能所必需的微量营养素.
- 准确量化WSV对于营养评估,疾病预防和药品质量控制至关重要.
- 在WSV分析中的挑战包括复杂的矩阵,维生素不稳定性,以及对标准化的方法的需求.
研究的目的:
- 综合审查WSV的样本准备和分析方法的进展和挑战.
- 在各种样本类型中探索WSV的各种分离,提取和检测技术.
- 突出纳米材料等新兴技术在增强 WSV 分析中的作用.
主要方法:
- 对样品制备技术的文献综述:酸/酶化水解,固相提取 (SPE),超声辅助提取 (UAE),索克斯莱特提取.
- 分析技术的分析:光谱测量,测量,HPLC,LC-MS,电化学方法
- 对提高灵敏度的纳米材料应用 (纳米粒子,量子点) 的讨论.
主要成果:
- 不同的提取和分离方法对WSV的确定具有不同的优点和局限性.
- HPLC和LC-MS是量化WSV的关键技术,纳米材料在色度和度测试中提高了灵敏度.
- 如矩阵效应和维生素稳定性等挑战需要仔细考虑可靠的结果.
结论:
- 讨论的方法显著提高了WSV分析的准确性和可靠性.
- 这篇评论为营养,临床和分析化学研究提供了宝贵的见解.
- 增强的WSV分析支持旨在预防维生素缺乏的公共卫生倡议.
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