Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

8.0K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
8.0K
Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Mitochondria01:37

Mitochondria

14.9K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
14.9K
Mitochondrial Membranes01:45

Mitochondrial Membranes

12.3K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
12.3K
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

340
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
340
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

35.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
35.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Synergistic antitumor activity of low-dose c-Met tyrosine kinase inhibitor and sorafenib on human non-small cell lung cancer cells.

Oncology letters·2018
Same author

Sorafenib inhibited cell growth through the MEK/ERK signaling pathway in acute promyelocytic leukemia cells.

Oncology letters·2018
Same author

Enhancing denitrification with waste sludge carbon source: the substrate metabolism process and mechanisms.

Environmental science and pollution research international·2018
Same author

Enhancement of malate production through engineering of the periplasmic rTCA pathway in Escherichia coli.

Biotechnology and bioengineering·2018
Same author

BMP4 facilitates beige fat biogenesis via regulating adipose tissue macrophages.

Journal of molecular cell biology·2018
Same author

CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis.

The Journal of clinical investigation·2018

相关实验视频

Updated: Sep 10, 2025

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.5K

线粒体DNA甲基化:分子机制和疾病影响的最新进展

Meng-Ting Yin1, Liang Guo1

  • 1Key Laboratory of Exercise and Health Sciences of the Ministry of Education, Shanghai University of Sport, Shanghai 200438, China; School of Exercise and Health and Collaborative Innovation Center for Sports and Public Health, Shanghai University of Sport, Shanghai 200438, China; Shanghai Key Lab of Human Performance, Shanghai University of Sport, Shanghai 200438, China; Shanghai Frontiers Science Research Base of Exercise and Metabolic Health, Shanghai University of Sport, Shanghai 200438, China.

Journal of advanced research
|August 23, 2025
PubMed
概括
此摘要是机器生成的。

线粒体DNA (mtDNA) 的甲基化模式如5mC,5hmC和6mA在疾病中至关重要. 先进的检测方法揭示了它们在线粒体功能障碍中的作用,

关键词:
5甲基细胞素5甲基细胞素MtDNA甲基化N6-甲基氨酸

更多相关视频

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.7K
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

25.7K

相关实验视频

Last Updated: Sep 10, 2025

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.5K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.7K
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

25.7K

科学领域:

  • 表观遗传学
  • 线粒体生物学
  • 分子医学

背景情况:

  • 线粒体DNA (mtDNA) 甲基化与各种病理有关,包括神经退行,心血管疾病,代谢障碍和衰老.
  • 失调的mtDNA甲基化可以通过影响mtDNA复制和转录来破坏细胞能量生产.
  • 尽管存在诸如核伪基因干扰等挑战,但先进的检测技术提高了mtDNA甲基化分析.

研究的目的:

  • 审查三个关键的mtDNA甲基化模式:5-甲基细胞素 (5mC),5-基甲基细胞素 (5hmC) 和N6-甲基氨酸 (6mA).
  • 总结它们存在的证据和疾病中的分子机制.
  • 提供有关mtDNA检测技术的最新进展的见解.

主要方法:

  • 专注于mtDNA甲基化模式的文献综述 (5mC,5hmC,6mA).
  • 对将mtDNA甲基化与疾病进展联系起来的分子机制的分析.
  • 对mtDNA甲基化检测新兴技术的评估.

主要成果:

  • 证据支持mtDNA中5mC,5hmC和6mA的存在和病理相关性.
  • mtDNA甲基化异常与线粒体功能受损和各种疾病有关.
  • 检测技术的进步正在改善mtDNA甲基化的研究.

结论:

  • 失调的mtDNA甲基化作为一种潜在的生物标志物和治疗点.
  • 了解mtDNA甲基化是阐明疾病中的线粒体功能障碍的关键.
  • 这种表观遗传洞察力促进了线粒体疾病的精准医学策略.