金属应力调节的细胞外聚合物使得Methylobacterium extorquens的增强和选择性吸附
Yun Liu1, Shujie Shang2, Bo Li3
1School of Materials Science and Engineering, Zhongyuan Critical Metals Laboratory, Zhengzhou University, Zhengzhou, Henan 450001, China; Zhongyuan Critical Metals Laboratory, Zhengzhou, Henan 450001, China; State Key Laboratory of Critical Metals Beneficiation, Metallurgy and Purification, Zhengzhou, Henan 450001, China.
Bioresource technology
|August 23, 2025
概括
细菌适应压力显著提高了的恢复. 这种方法增强了微生物细胞外聚合物 (EPS) 的优质吸附性,为稀土元素提取提供了更绿色的替代方案.
科学领域:
- 生物技术
- 环境科学
- 材料科学
背景情况:
- 由于的分布和昂贵,对环境有影响的传统方法,的回收具有挑战性.
- 微生物细胞外聚合物 (EPS) 具有作为生物吸收剂的潜力,但在复杂混合物中缺乏特异性.
研究的目的:
- 通过诱导细菌的向应激来增强使用EPS的选择性吸附.
- 在应力适应的EPS中研究改善吸附的机制.
主要方法:
- 在压下培养的Methylobacterium extorquens产生适应菌株 (Mex4).
- 适应菌株及其EPS的评估吸附能力和选择性.
- 使用FTIR,3D-EEM,氨基酸和蛋白质组分析进行了EPS的表征.
主要成果:
- 适应应力 (Mex4) 的吸收能力增加了三倍 (79 mg/g).
- EPS的吸附能力为220 mg/g,具有很高的选择性 (分离因子高达38.5).
- 在EPS中增加的功能组 (thiol, carboxyl) 和类似蛋白质的成分 (cysteine, glutamic acid, glycine) 与增强的金属结合有关.
结论:
- 金属诱导的压力是改造微生物EPS以改善吸附的有效策略.
- 这种方法为有效和选择性回收关键稀土元素,如提供了有希望的途径.
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