肠道细菌转移促进DIO小鼠中的β细胞功能障碍
Xiang-Fang Yu1,2, Chetali Gurung2, Zhongjia Yu2
1Department of Endocrinology, Shenzhen Children's Hospital, Shenzhen, China.
Scientific reports
|August 23, 2025
概括
肠道细菌转移到胰腺会导致2型糖尿病 (T2DM) 的恶化. 减少这种细菌迁移改善了小鼠模型中的葡萄糖控制和胰腺功能,突出显示了肠道微生物群和T2DM之间的联系.
科学领域:
- 微生物学
- 代谢疾病
- 医疗技术
背景情况:
- 越来越多的证据将肠道微生物与胰岛素抵抗和2型糖尿病 (T2DM) 联系在一起.
- 需要直接了解肠道菌群在T2DM进展中的作用.
- 肠道细菌转移 (IBT) 是连接肠道失调与胰腺功能障碍的潜在机制.
研究的目的:
- 精确评估肠道细菌从肠道转移到胰腺的变化.
- 在2型糖尿病中研究IBT与胰腺功能障碍之间的病理关系.
- 评估深度学习辅助的IBT跟踪方法的实用性.
主要方法:
- 使用现场光杂交 (FISH),16S rRNA amplicon测序和深度学习辅助方法.
- 使用饮食诱导的肥胖 (DIO) 和抗生素诱导的微生物群破坏 (AIMD) - DIO小鼠模型.
- 在胰腺中量化细菌负荷和评估胰腺功能和葡萄糖平衡.
主要成果:
- 深度学习提高了细菌追踪的准确性和客观性.
- DIO小鼠显示IBT增加,与肠道脂质积累和屏障损害有关.
- 在DIO小鼠中,增加的胰腺细菌负荷与恶化的葡萄糖代谢和胰岛素分泌相关.
- 在AIMD- DIO小鼠中,IBT降低,岛屿结构保持,葡萄糖平衡改善.
- 在转移的细菌数量和T2DM严重程度之间发现了强烈的相关性.
结论:
- 提供从肠道到胰腺的细菌迁移的客观证据.
- 在IBT,胰腺功能障碍和T2DM之间建立了病理关系.
- 展示了AI技术评估IBT及其在代谢疾病中的作用的潜力.
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