尼古丁胺单核酸在化疗期间保护卵巢功能和卵细胞发育能力
Lin Shen1,2,3, Hemei Li4, Xueqi Gong1
1Reproductive Medicine Center, Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
尼古丁胺单核酸 (NMN) 保护卵巢功能免受化疗损伤. 这项研究表明,NMN通过减少DNA损伤和亡来保护生育能力,为癌症患者提供潜在的非侵入性生育能力保护策略.
科学领域:
- 生殖生物学
- 癌症学
- 生物化学
背景情况:
- 化疗,特别是环胺 (CTX),可能导致女性癌症患者的卵巢功能障碍和不孕症.
- 现有的生育保护方法有局限性, 需要新的非侵入性策略.
- 尼古丁胺氨基二核酸 (NAD+) 在DNA修复和亡中起作用,使其前体成为卵巢保护的潜在候选物.
研究的目的:
- 调查尼古丁胺单核酸 (NMN) 的保护作用,一个NAD+前体,对循环胺 (CTX) 诱导的卵巢损伤.
- 评估NMN在化疗期间作为一种非侵入性生育保护策略的潜力.
主要方法:
- 雌性C57小鼠接受盐水,CTX或CTX与NMN补充剂14天.
- 评估了卵巢储备,卵细胞质量和胚胎发育.
- 在卵巢组织和胚囊中测量了NAD+水平,反应性氧物种 (ROS),DNA损伤和亡.
主要成果:
- 补充NMN增加了卵巢NAD+水平,改善了卵巢储备,并增强了内分泌功能.
- 在卵巢组织和胚囊中,NMN减少了ROS,DNA损伤和亡.
- 胚胎发育率 (双细胞和胚胎细胞形成) 和胚胎细胞数量在NMN治疗中得到改善.
结论:
- 对CTX诱导的生殖毒性有显著的保护作用.
- 在接受化疗的癌症患者中,NMN 是一种有前途的非侵入性策略.
- 这些保护机制可能涉及Banp,Rbm47和Sgk1等关键基因.
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