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在胚胎发育的后期阶段对转录基因特征的新见解

Di Zhao¹, Yifan Shi¹, Jiatai Deng¹

¹College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Engineering Research Center of Poultry Production Safety, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, China.

Poultry science

|August 24, 2025

概括

这项研究揭示了胚胎肌肉发育的关键遗传调节者,确定了在化过程中影响生长和能量代谢的特定微RNA和编码基因. 这些发现为推进肉和本地品种肌肉发育差异的分子机制提供了洞察力.

科学领域:

鸟类物种的比较基因组学和转录组学.
胚胎肌肉发育和生长的分子机制.
在发育过程中非编码RNA (lncRNA,circRNA,microRNA) 的调节作用.

背景情况:

对于关键化阶段的肌肉发育,有有限的综合转录学数据.
在Arbor Acres (AA) 肉和TaoYuan (TY) 之间观察到明显的肌肉生长和纤维特性差异.
了解这些差异的分子基础对于家禽的繁殖和发展至关重要.

研究的目的:

在晚期胚胎发育 (E17-E21) 期间对AA和TY的胸肌进行编码和非编码RNA概况的综合分析.
识别关键基因和参与肌肉发育的调节网络.
阐明微RNA,lncRNA和circRNA在这些发育过程中的作用.

主要方法:

对编码和非编码RNA测序数据 (microRNAs,circRNAs,lncRNAs) 的综合分析.
差异表达分析以识别显著改变的转录.
权重基因共同表达网络分析 (WGCNA) 以确定关键的调节基因.
使用miRanda预测构建竞争的内源RNA (ceRNA) 网络.

主要成果:

鉴定了4,577个差异表达的基因 (DEG),143个差异表达的微RNA (DEM),90个差异表达的circRNA (DEC) 和3,159个差异表达的lncRNA (DEL).
优先考虑五个编码基因 (FOSL2,PDE4B,TRIB1,THBS1,FBXO32) 作为肌肉发育的潜在关键调节者.
在TY中显著激活葡萄糖分解/葡萄糖生成途径,可能支持的能量需求.
构建了通过lncRNA和circRNA海绵调节发育基因的四个微RNA (gga-miR-206,gga-miR-383-3p,gga-miR-449a,gga-miR-449c-5p) 的ceRNA网络.

结论:

这项研究提供了两种不同的品种胚胎肌肉发育的综合转录图.
确定了可能调节肌肉生长和能量代谢的关键编码基因和微RNA.
构建的ceRNA网络为控制鸟类肌肉发育的复杂的转录后调节机制提供了新的见解.

关键词:

小竞争的内源性RNA 胚胎的发育转录组形状

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