通过与A1CF蛋白结合,CircMTND5参与狼性炎中的管间损伤
Cong Ma1, Junjun Luan1, Congcong Jiao1
1Department of Nephrology, Shengjing Hospital of China Medical University, Shenyang, China.
International immunopharmacology
|August 24, 2025
概括
循环RNA MTND5 (circMTND5) 通过与RNA结合蛋白A1CF结合,可以缓解狼性炎 (LN) 管间纤维化. 这种相互作用有助于调节LN进展中的纤维化标志物.
科学领域:
- 肝脏病学
- 分子生物学
- 免疫学
背景情况:
- 狼性炎 (LN) 是系统性红斑狼的一个常见并发症.
- 通过与miR6812相互作用,循环RNA MTND5 (circMTND5) 参与了LN的发病.
- 与circMTND5相互作用的RNA结合蛋白 (RBPs) 在LN中的作用尚不清楚.
研究的目的:
- 研究circMTND5和RBPs在LN进展中的相互作用.
- 澄清 circMTND5 在 LN 病变的潜在机制.
- 要确定circMTND5是否影响LN中的管间纤维化.
主要方法:
- 使用免疫染,西部染和qPCR分析LN患者和小鼠的样中的蛋白质和circRNA表达.
- 在人类细胞中,RNA免疫沉 (RIP) 测定证实了circMTND5与A1CF的结合.
- 在人类细胞中进行的抑制和过度表达实验调节了MTND5水平,以评估其功能影响.
主要成果:
- A1CF表达在LN脏下降并局部化到管状上皮细胞.
- 在细胞中,circMTND5的破坏导致E-cadherin的减少和α-SMA,TGF-β和纤维素的增加.
- 过度表达circMTND5逆转了TGF-β诱导的纤维变化.
结论:
- circMTND5 与RNA结合蛋白A1CF结合.
- 在狼性炎中,circMTND5可缓解管间纤维化.
- 循环MTND5-A1CF相互作用是控制LN进展的潜在机制.
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