一种改进和经济的LLE-LC/MS方法来量化酸乙醇:在临床酒精测试中识别和分离同位素交叉谈话
Naga Veera Yerra1, Christopher Pini1, Carlos Cordon-Cardo1
1Clinical Chemistry Service, Department of Pathology, Molecular and Cell-Based Medicine, The Mount Sinai Hospital, New York 10029 NY, USA.
Clinical biochemistry
|August 24, 2025
概括
这项研究开发了一种新的LC-MS/MS方法,用于准确测量长期酒精使用的酸乙醇 (PEth) 生物标志物. 经过验证的试验为临床环境提供了可靠的高吞吐量量化.
科学领域:
- 临床化学
- 分析化学
- 生物标志物发现
背景情况:
- 酸乙醇 (PEth) 是酒精消耗的生物标志物.
- 在红细胞中稳定3 - 4周.
- 精确量化PEth对于监测酒精使用至关重要.
研究的目的:
- 为量化PEth 16:0/18:1 (POPEth) 和PEth 16:0/18:2 (PLPEth) 开发和验证一个强大的高通量LC-MS/MS方法.
- 解决PEth量化中的分析挑战,包括同位素交叉交谈和染色干扰.
主要方法:
- 已建立的液体-液体提取与优化的LC-MS/MS参数.
- 验证了该方法的线性,精度,分析范围,灵敏度,特异性,转移,稀释线性,稳定性,矩阵效应和回收.
- 与外部参考实验室进行方法比较.
主要成果:
- 该方法显示了POPEth和PLPEth的优异线性 (r2=0.99) 和精度 (CV ≤10%).
- 获得78-85%的回收率,并解决了同位素交叉谈话,没有转移.
- 与外部实验室有很强的相关性 (r2=0.99).
结论:
- 已成功开发和验证了一种敏感,特定和稳固的LC-MS/MS方法来量化POPEth和PLPEth.
- 该试验可以改善恢复,消除交叉谈话,并适用于高通量临床分析.
- 这种方法可以在日常实践中可靠且具有成本效益地监测酒精消费.
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