在暴露于微粒物质的斑马鱼发育过程中出现类似自闭症谱系障碍的行为
Shayla Victoria1, Courtney Roper1
1University of Mississippi, Department of BioMolecular Sciences, United States.
空气污染,特别是颗粒物 (PM),并没有显著改变与自闭症谱系障碍 (ASD) 相关的斑马鱼行为. 在这种动物模型中,黑素没有改善PM诱导的行为变化.
科学领域:
- 环境健康
- 神经发育障碍
- 毒理学
背景情况:
- 自闭症谱系障碍 (ASD) 是一种由遗传和环境因素影响的神经发育状况.
- 空气污染,特别是颗粒物,越来越多地被认为是自闭症的潜在环境风险因素.
- 在ASD中暴露的特定行为表型在很大程度上仍未被描述.
研究的目的:
- 在斑马鱼模型中研究颗粒物 (PM) 暴露的行为影响.
- 探索黑素对PM诱导的行为变化的潜在缓解作用.
- 将PM诱导的行为与酸 (VPA) 诱导的行为进行比较,这是用于模拟ASD的已知原体.
主要方法:
- 斑马鱼幼虫从受精后6小时暴露在PM标准参考物质 (SRM1649b) 和黑素中,持续5天.
- 进行了13天的行为测试,以量化与自闭症相关的行为.
- 用瓦尔酸 (VPA) 作为阳性对照剂进行比较.
主要成果:
- 暴露于颗粒物 (PM) 并没有诱导与斑马鱼的VPA暴露相似的行为模式.
- 对PM和VPA的联合暴露没有产生添加或协同的行为效应.
- 在PM或VPA暴露后观察到的大部分行为变化未能得到改善.
结论:
- 单独暴露于颗粒物 (PM) 并没有在斑马鱼模型中复制与ASD相关的行为.
- 在该模型中,黑素对缓解PM或VPA诱导的行为变化无效.
- 需要进一步研究以了解PM等环境因素与自闭症神经发育结果之间的复杂相互作用.
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