蛋白质组学和单细胞剖析确定质素驱动的先前免疫影响 肺状癌 新辅助疗法
Jiangnan Zhao1, Mo Shen1, Xia Xu2
1Department of Respiratory and Critical Care Medicine, Center for Oncology Medicine, The Fourth Affiliated Hospital of School of Medicine, International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China; Zhejiang Key Laboratory of Precision Diagnosis and Treatment for Lung Cancer, Yiwu, China.
Cancer letters
|August 24, 2025
概括
瘤的角质化预测了对肺癌免疫疗法的反应. 较高的质化表明耐药性,而较低的质化表明更好的结果和免疫细胞透.
科学领域:
- 癌症学
- 免疫疗法
- 生物标志物发现
背景情况:
- 肺状细胞癌 (LUSC) 对新辅助免疫检查点阻塞的反应不同.
- 需要预测生物标志物来指导LUSC治疗决策.
研究的目的:
- 在LUSC中确定预测免疫检查点阻塞反应的生物标志物.
- 研究瘤角质化在治疗耐药性和患者结局中的作用.
主要方法:
- 在470名LUSC患者的血清CYFRA21-1和CK5/6免疫组织化学分析.
- 对167个未经治疗的活检进行蛋白质分析,以分层患者.
- 单细胞RNA测序以分析瘤微环境和细胞状态.
主要成果:
- 血清CYFRA 21-1和高CK5/6表达与非完全的病理反应和残留瘤负担相关.
- 低角质化 (KRT_L) 的患者表现出增强的免疫透,较低的残留瘤和更高的生存率.
- 高质化 (KRT_H) 瘤表现出质蛋白增加,代谢激活,茎状特征和免疫抑制.
结论:
- 瘤角质化是LUSC治疗耐药性的关键决定因素.
- 角质化与免疫寒冷的微环境,癌症干性和免疫疗法的效果受损有关.
- 基于质素的分层可以为LUSC患者提供个性化治疗.
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