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喉状细胞癌中淋巴结转移相关的微生物特征

Fangxu Yan¹, Shibo Chen^2,3,4, Xin Xia¹

¹Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Wangfujing, Beijing, 100730, China.

Scientific reports

|August 24, 2025

概括

喉平细胞癌 (LSCC) 的微生物组变化与淋巴结转移相关. 口腔微生物组分析提供了一种潜在的非侵入性方法来检测LSCC患者的隐性转移.

科学领域:

癌症学
微生物学
基因组学

背景情况:

淋巴结 (LN) 转移是喉平细胞癌 (LSCC) 的关键预后因素.
微生物组在癌症进展中的作用是一个新兴的研究领域.
了解微生物与转移的关联可以帮助患者分层和治疗策略.

研究的目的:

在LSCC中调查与淋巴结转移相关的微生物特征.
根据转移状态来分层LSCC患者的这些微生物特征的潜力.
评估口腔微生物组作为隐性淋巴结转移的非侵入性生物标志物的有用性.

主要方法:

使用16S rRNA基因测序分析了108名LSCC患者的瘤组织,相邻的正常组织,淋巴结和口腔冲洗中的微生物组 (36 LN+,72 LN-).
使用PICRUSt2预测了微生物的功能潜力.
基于分层交叉验证的随机森林模型被用于识别转移相关的微生物属.

主要成果:

在LN+和LN- LSCC瘤组织中观察到显著的微生物组成差异,在LN- 病例中Ralstonia富含LN+和Fusobacterium.
功能预测表明LN+患者的脂质生物合成,能量代谢和细胞壁合成途径的丰富,特别是在瘤和口腔冲洗样本中.
基于瘤,淋巴结和口腔微生物群的分类器在区分LN+和LN-患者方面表现出显著的准确性,淋巴结分类器的准确性达到84. 31%.

结论:

在LSCC中,微生物组的改变与淋巴结转移有关.
口腔微生物组作为检测隐性淋巴结转移的非侵入性生物标志物具有潜力.
需要对更大群体进行进一步的验证,以确认这些初步发现.

关键词:

16S rRNA 的使用生物标志物喉状细胞癌淋巴结转移微生物组

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