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检查基于杂交的 siRNA 分析物的 LC-MS 方法

Karan Agrawal¹, Wenying Jian¹, Long Yuan²

¹Bioanalysis Discovery & Development Sciences, Johnson & Johnson, Spring House, PA, USA.

|August 25, 2025

概括

基于混合的LC-MS为分析寡核酸治疗方法提供了敏感和特定的方法. 本审查评估了其用于小干扰RNA (siRNA) 的应用,并讨论了监管用途的未来改进.

科学领域:

生物化学
分析化学
药理学

背景情况:

寡核酸治疗药物,包括抗意义寡核酸 (ASO) 和小干扰RNA (siRNA),是一个不断增长的药物类别.
这些分子的生物分析对于治疗开发和监管批准至关重要.
基于混合的液体染色体质谱 (LC-MS) 正成为一种强大的寡核酸分析工具.

研究的目的:

审查基于杂交的 siRNA 生物分析的 LC-MS 的进展和挑战.
在样本准备和分析过程中确定需要改进的领域.
将基于杂交的LC-MS与其他寡核酸生物分析工作流进行比较,并评估其调控潜力.

主要方法:

这项研究重点是基于杂交的LC-MS技术进行寡核酸分析.
它包括评估和优化样品准备和分析步骤.
与现有的生物分析工作流进行比较分析.

主要成果:

基于混合的LC-MS为寡核酸分析提供了高灵敏度和特异性.
由于感应链竞争导致的siRNA分析的挑战已得到解决.
该平台显示了简化工作流程和增强信息恢复的潜力.

结论:

基于混合的LC-MS是一种有前途的生物分析平台,用于寡核酸治疗,特别是siRNA.
进一步优化可以提高效率并扩大其监管提交的实用性.
这种技术将成为支持药物开发的前线试验.

关键词:

混合化在LC-MS 捕获探测器核酸小干扰RNA (siRNA) 的

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