量化时间动力学 卡斯特盆地中的病原体度和定量微生物风险评估
Shishir K Sarker1, Ryan T Dapkus1, Diana M Byrne2
1Department of Earth and Environmental Sciences, University of Kentucky, Lexington, KY 40506, USA.
Water
|August 25, 2025
概括
像皇家泉水一样,在潮湿的天气中大肠杆菌的含量更高,这表明便污染的风险. 定量微生物风险评估表明,职业和休接触的感染概率很高.
科学领域:
- 环境科学
- 微生物学
- 水资源管理
背景情况:
- 岩层是重要的水源,但由于集成排水而容易受到污染.
- 肯塔基州的皇家泉盆地为33,000多名客户提供服务, 经历了城市和农业用地影响.
- 了解岩系统中的病原体动态对于公众健康至关重要.
研究的目的:
- 在不同的天气条件下检查皇家泉水中的大肠杆菌 (E. coli) 动态.
- 使用定量微生物风险评估 (QMRA) 估计与大肠杆菌O157:H7暴露相关的潜在健康风险.
- 确定影响石水系统健康风险的关键因素
主要方法:
- 从2021年6月到2022年6月每周取水样并进行大肠杆菌度分析.
- 在潮湿和干燥的天气期间对大肠杆菌水平进行比较.
- 使用职业和休接触场景的文献数据进行量化微生物风险评估 (QMRA).
主要成果:
- 大肠杆菌度在12至1732. 8MPN/100 mL之间,几何平均值为117. 2MPN/100 mL.
- 与干旱时期相比,湿期间的大肠杆菌平均度大约是两倍.
- 职业接触的年均感染概率为5. 11 × 10^-3,休接触的年均感染概率为1. 45 × 10^-2.
结论:
- 尽管qPCR没有检测到特定的病原体大肠杆菌O157:H7,但大肠杆菌的存在表明皇家春季可能存在便污染.
- 病原体对大肠杆菌的比率和摄入率对健康风险很敏感,强调需要准确的风险因素数据.
- 持续监测和探索各种检测方法对于管理脆弱的石水系统的健康风险至关重要.
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