具有自动图像分析的3D多组织微生理系统用于抗癌和心脏毒性药物查
Edgar A Borrego1,2, Jose L Perez1,2, Aibhlin Esparza1,2
1Department of Metallurgical, Materials, and Biomedical Engineering (MMBME), The University of Texas at El Paso (UTEP), 500 W University Ave, 79968 El Paso, TX, USA.
Discover applied sciences
|August 25, 2025
概括
这项研究开发了用于体外药物测试的3D微生理系统 (MPS). 这种新型的微流体平台准确地模拟了癌症和心脏毒性,有助于临床前药物查.
科学领域:
- 生物医学工程
- 癌症研究
- 药理学
背景情况:
- 微生理系统 (MPS) 为研究细胞反应提供受控的体外环境.
- 三维组织模型对于模拟体内条件和改善药物疗效研究至关重要.
- 目前在复杂组织中评估化疗效果的方法需要改进.
研究的目的:
- 开发和验证用于体外化疗药物疗效测试的新型3D微流体系统.
- 模拟像乳腺癌和质母细胞瘤这样的恶性瘤, 并评估化疗引起的心脏毒性.
- 整合自动化图像分析以进行细胞对治疗反应的定量评估.
主要方法:
- 开发用于3D组织培养的双腔闭环微流体平台.
- 在MPS中共培养HER2+乳腺癌和心脏细胞,以及质母细胞瘤模型.
- 使用基于Python的自动图像分析脚本 (AIAPS) 来从3D光图像中量化细胞大小.
主要成果:
- 3D MPS 保持了血统特异性的生物标志物表达和生理相关的细胞形态.
- 在组织模型中显示化疗后细胞大小的可检测变化.
- 成功模拟化疗引起的心脏毒性和质母细胞瘤药物反应.
结论:
- 开发的3D MPS是一个多功能且有效的临床前药物查平台.
- 该系统可对细胞对化疗剂的反应进行可重现的量化.
- 这项技术有望推进个性化医疗和降低药物开发成本.
更多相关视频
10:37Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
Published on: March 14, 2021
6.7K
12:41Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Published on: December 23, 2022
5.0K
相关概念视频
01:13Radiological Investigation III: Pulmonary Angiogram and PET Scan
Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
01:20Imaging Studies for Cardiovascular System III: X-Ray
The most common cardiovascular diagnostic test is an X-ray. It produces images of the heart, blood vessels, and adjacent structures.
Definition and Purpose
An X-ray, or radiograph, is a non-invasive method that uses ionizing radiation to take images of internal structures. It is mainly used in cardiac imaging to examine the heart, lungs, and major blood vessels, aiming to identify abnormalities in the heart's size, shape, and position, such as heart failure, congenital defects, and vascular...
Definition and Purpose
An X-ray, or radiograph, is a non-invasive method that uses ionizing radiation to take images of internal structures. It is mainly used in cardiac imaging to examine the heart, lungs, and major blood vessels, aiming to identify abnormalities in the heart's size, shape, and position, such as heart failure, congenital defects, and vascular...