在3D挤出生物打印中,水力动态剪切应力对藻类细胞命运的影响
Md Shihab Shakur1, Emily Lazarus2, Can Wang3
1School of Industrial Engineering and Management, Oklahoma State University, Stillwater, OK 74078, USA.
概括
3D生物打印需要仔细控制剪切压力. 藻类功能在5kPa以上受损,而生存能力在14kPa以上不可逆转地下降,影响生物传感和生物燃料生产等应用.
科学领域:
- 生物技术
- 生物材料工程
- 细胞工程
背景情况:
- 水生光合成生物的3D生物打印在生物传感,废水处理和生物燃料生产方面具有潜力.
- 生物打印矩阵中的藻类固定是可行的,但影响细胞功能和活力的水力动力压力值仍未得到充分研究.
研究的目的:
- 在3D生物打印过程中研究水力动力切割应激对Chlamydomonas reinhardtii细胞的功能和活力的影响.
- 在生物打印过程中切割应激水平与随后的细胞命运之间建立相关性.
主要方法:
- 基于纳维埃-斯托克斯方程的计算流体动力学 (CFD) 模型的开发,以预测挤出过程中的剪切应力.
- 进行并行细胞培养实验,以评估生物打印结构中的藻类细胞功能,生长率和生存能力.
主要成果:
- 藻类的细胞功能对剪切压力比细胞活力更敏感.
- 在5kPa左右的最大剪切应力下观察到功能障碍,但对生存能力没有影响.
- 在14kPa以上,功能和可行性均出现显著且不可逆转的下降.
结论:
- 3D生物打印中的细胞活力和功能受到过程参数的显著影响,特别是剪切应力.
- 在生物打印后评估生命力和功能至关重要,因为功能障碍可能会导致生命力丧失.
- 优化生物打印参数以尽量减少剪切压力对于维持藻类细胞在生物技术应用中的性能至关重要.
相关概念视频
Pressure of Fluids
13.0K
There are many examples of pressure in fluids in everyday life, such as in relation to blood (high or low blood pressure) and in relation to weather (high- and low-pressure weather systems). A given force can have a significantly different effect, depending on the area over which the force is exerted. For instance, a force applied to an area of 1 mm2 has a pressure that is 100 times greater than the same force applied to an area of 1 cm2. That's why a sharp needle is able to poke through...
13.0K
Shearing Stress
2.5K
Shearing stress, denoted by the Greek letter tau (τ), is stress caused by forces acting transversely on an object. These forces create internal ones within the entity in the plane where the external forces are applied. The resultant of these internal forces is the shear in the section.
The average shearing stress can be calculated by dividing the shear by the area of the cross-section.
The average shearing stress can be calculated by dividing the shear by the area of the cross-section.
2.5K
Osmotic Pressure
299
Osmosis is a process where solvent molecules move toward a solution through a semipermeable membrane. As the solution dilutes due to the entry of solvent, it expands. This expansion increases the hydrostatic pressure of the solution. When the hydrostatic pressure equals the osmotic pressure, osmosis stops.Osmotic pressure, denoted by Π, is the minimum pressure needed to prevent the solvent from passing into the solution by osmosis. The van 't Hoff equation calculates the osmotic pressure...
299
Pressure Variation in a Fluid at Rest
1.1K
In a fluid at rest, the pressure at any point beneath the fluid surface depends solely on the depth, not on the container's shape or size. This principle, known as hydrostatic pressure, arises because, in stationary fluids, there is no acceleration, meaning the forces within the fluid balance out. Only vertical forces, caused by the weight of the fluid above, contribute to pressure changes with depth.
When measuring pressure at two different levels within the fluid, the difference in...
When measuring pressure at two different levels within the fluid, the difference in...
1.1K


