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Updated: Sep 10, 2025

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活细胞中的化-皮克特-斯格勒反应

Kaleena Basran1, Nathan W Luedtke1,2

  • 1Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montréal, Québec, H3A 0B8, Canada.

Angewandte Chemie (International ed. in English)
|August 25, 2025
PubMed
概括

研究人员开发了一种新的光探测器FLipA-HIPS,用于检测活细胞中的脂性化物. 这种探测器提供了一种敏感和选择性的方法,用于可视化细胞环境中的氧化应激标志物.

关键词:
化物生物成像化反应脂质氧化应激

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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