脊椎动物基因组内源性逆转录病毒的多样性,进化和转录
Zhi-Jian Zhou1, Yang Xiao1, Jie Fang1
1Hunan Provincial Key Laboratory of Medical Virology, Institute of Pathogen Biology and Immunology, College of Biology, Hunan University, 27 Tianma Rd., Changsha, Hunan, 410012, China.
概括
这项研究全面描述了蝙蝠内生逆转录病毒 (ERV) 和ERV类元素 (ERVLEs),揭示了它们在蝙蝠基因组中的进化历史和潜在功能. 发现突出了它们在蝙蝠基因组进化和潜在基因调节中的作用.
科学领域:
- 病毒学
- 基因组学
- 进化生物学
背景情况:
- 蝙蝠 (Chiroptera) 是动物感染病毒的重要储存库.
- 内生逆转录病毒 (ERV) 可以影响宿主基因表达和生理学.
- 蝙蝠ERV和ERV类元素 (ERVLEs) 在很大程度上仍然没有特征.
研究的目的:
- 在61种蝙蝠中系统地探索和描述ERV和ERVLE.
- 研究蝙蝠ERV的进化动态,类型和潜在功能.
- 为未来的蝙蝠ERV研究提供基础数据.
主要方法:
- 对61种蝙蝠的ERV和ERVLE序列进行生物信息分析.
- 蝙蝠ERV和蝙蝠ERVE的识别和分类
- 使用蝙蝠转录组估计ERV整合时间和转录潜力的分析.
主要成果:
- 确定了10352个蝙蝠ERV和5884个蝙蝠ERVLEs,包括完整的deltaretrovirus序列和复合物.
- 估计蝙蝠-ERV融合主要发生在过去的1000万到400万年间,表明长期的共同进化.
- 在各种蝙蝠组织中发现了许多蝙蝠ERV和蝙蝠ERVEs的转录潜力,这表明了活跃的基因表达和潜在的功能.
结论:
- 本研究提供了蝙蝠ERV和ERVLE的全面描述.
- 蝙蝠ERV在形成蝙蝠基因组和宿主病原体共同进化的过程中发挥着重要作用.
- 活跃表达的蝙蝠ERV和ERVLE可能含有功能基因,对蝙蝠生理和病理有影响.
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