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Updated: May 10, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
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一种高产化学蛋白质合成的优雅的一结合脱硫方法

Vishal Malik1, Abhisek Kar1, Anandh Muthiah Venkatachalam1

  • 1Tata Institute of Fundamental Research Hyderabad, 36/p Gopanpally, Hyderabad, Telangana, 500046, India.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 25, 2025
PubMed
概括

通过结合原生化学结合和脱硫, 一种新的单方法可实现有效的化学蛋白质合成. 这种方法使用乙和N-乙囊来克服先前方法的局限性,简化了蛋白质的生产.

关键词:
化学蛋白质合成脱硫原生化学结合一个固相合成

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...