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通过巨细胞免疫调节促进伤口愈合的 rhGM-CSF 作用

Xiaoling Zhang^1,2,3,4, Zhiyi Zhao^1,3,4, Song Gong^1,3,4

¹Division of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The Journal of clinical endocrinology and metabolism

|August 25, 2025

概括

在小鼠和人类中,重组人粒细胞巨细胞殖民地刺激因子 (rhGM- CSF) 加快伤口愈合. 这种生长因子通过增加血管化和转移巨子向抗炎M2表型来促进愈合.

科学领域:

复原医学
创伤治愈研究
免疫学

背景情况:

重组人粒细胞巨细胞殖民地刺激因子 (rhGM-CSF) 主要用于血液学疾病.
新出现的证据表明rhGM-CSF在促进伤口修复方面的潜力.
本研究研究了rhGM-CSF在糖尿病和非糖尿病伤口愈合中的有效性和潜在机制.

研究的目的:

验证rhGM-CSF对动物模型和人类受试者的伤口愈合的治疗效果.
阐明rhGM-CSF影响伤口愈合过程的分子机制,特别是巨分化.

主要方法:

在C57BL/6J小鼠中创建了全厚伤口,并用局部的rhGM-CSF或盐水处理.
一项临床试验将糖尿病下肢患者随机分为rhGM-CSF干预组或对照组.
使用HE染色,免疫光和西部斑点分析伤口组织,以评估巨细胞亚型和CD31和VEGF等分子标记物.

主要成果:

在小鼠 (2.53±0. 25 mm2/天) 和人类 (97. 7±8. 9% 的面积减少) 中,局部的rhGM-CSF显著加快了伤口的关闭.
治疗 rhGM-CSF 增加了 CD31 和 VEGF 表达,这表明血管生成增强.
这种干预导致了从M1转向M2巨细胞的显著转变,M2a和M2d亚型在不同的时间点占主导地位.

结论:

局部的rhGM-CSF有效地促进糖尿病和非糖尿病伤口的愈合.
这种治疗效应部分由巨细胞对M2亚型的偏向介导.
rhGM-CSF是加速伤口修复的有希望的治疗药物.

关键词:

血管新生糖尿病巨细胞 rHGM-CSF的使用情况

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