在人类肠道中存在的细菌和真菌物种之间的共享代谢
Haley Gause1, Alexander D Johnson1,2
1TETRAD Graduate Program, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158.
概括
菌种Candida albicans和细菌Enterococcus faecalis参与肠道内的代谢循环. 通过C. albicans的酸盐和酸盐排毒,促进了两种微生物的生长.
科学领域:
- 微生物学
- 微生物生态学
- 系统生物学
背景情况:
- 菌和菌是人类肠道微生物群的关键成员.
- 了解物种间的相互作用对于解读肠道生态系统的动态至关重要.
研究的目的:
- 在共同培养过程中研究C. albicans和E. faecalis之间的转录反应和代谢相互作用.
- 解释它们在肠道环境中的共存机制.
主要方法:
- 在单种和共同种植中使用双重RNA测序来分析两种物种的基因表达.
- 实验是在试验室和小鼠肠道模型中进行的.
- 功能分析证实了酸盐交叉养和甲排毒的作用.
主要成果:
- 在C. albicans和E. faecalis中,共同培养诱导了显著的转录变化.
- 酸盐代谢基因受到上调,C. albicans产生酸盐,E. faecalis利用它,增强E. faecalis的生长.
- C. albicans高调格式脱酶基因,使E. faecalis产生的有毒格式脱成为可能,从而赋予了生长优势.
结论:
- 一个涉及酸盐交叉养和酸盐排毒的代谢循环促进了C. albicans和E. faecalis的共同生长.
- 这些发现突显了代谢相互作用在肠道微生物组结构中的重要性.
- 这项研究揭示了肠道中一种新的物种间代谢共生.
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