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猪中氨基酸代谢的年龄相关变化和与肠道微生物群的相关性

Caiyun You¹, Xiaoyi Long¹, Haiyang Wei¹

¹Research Center for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing, 400715, P.R. China.

The Journal of nutrition

|August 25, 2025

概括

猪肝和肠道氨基酸代谢随着年龄的增长而发生变化. 肠道微生物与代谢物相关联,而肝脏甲酸循环酶和mTORC1信号减少.

科学领域:

动物生理学
营养生物化学
胃肠道代谢

背景情况:

了解猪肝脏和肠道中的氨基酸代谢对于通过营养策略提高利用效率至关重要.
年龄相关的代谢变化对营养处理和整体动物健康有重大影响.

研究的目的:

研究生长猪肝脏和肠道组织中与年龄相关的氨基酸代谢变化.
确定猪年龄影响的主要代谢途径和微生物关联.

主要方法:

在五个不同年龄 (90,120,150,180,210天) 的猪中,
分析包括新陈代谢酶活性,基因和蛋白质表达,微生物群组成,肝脏,膜和肠道含量中的代谢量.
统计分析使用SAS 9.2线性/平方模型来确定年龄依赖的效应.

主要成果:

随着年龄的增长,特别是在第210天,肝脏甲酸盐循环酶 (甲酸合成酶-I,甲酸转糖酶) 的活性和甲酸复合物1 (mTORC1) 信号的标下降.
随着年龄的增长,膜粘膜酶 (谷氨酸脱酶,氨酸转移酶,酸转移酶) 的活性增加,在210日与90日相比,其水平更高.
肝脏代谢学发现31种不同丰富的代谢物,富含酸盐/ 酸盐代谢物,并发现特定的酸盐/ 酸盐代谢物与Temporobacter等结肠微生物之间的相关性.

结论:

随着年龄的增长,杰纳的谷氨酸循环酶活动增加,与肝脏甲基氨酸循环酶活性和mTORC1表达的下降形成对比.
特定的结肠细菌,如Temporobacter,与宿主谷氨酸和氨酸代谢物有显著的关联,这表明肠道微生物群-代谢物轴.
这些年龄相关的新陈代谢变化凸显了对营养和肠道健康的重要生理变化.

关键词:

氨基酸代谢谷氨酸循环养殖后的猪甲素循环

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