自体血小板丰富的双网络水凝促进人类子宫内膜再生
Xiao Chen1, Chengcheng Zhu1, Lu Wang1
1Center for Reproductive Medicine, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, 322000, China.
Acta biomaterialia
|August 25, 2025
概括
一种新型的双网络水凝有效地提供富血小板血 (PRP),用于治疗子宫内粘附 (IUA). 这种增强的PRP输送通过激活WNT信号通路促进子宫内膜修复和再生.
科学领域:
- 生物材料科学
- 复原医学
- 妇科医生
背景情况:
- 宫内粘附 (IUA) 对生育和生殖健康构成挑战.
- 目前使用富血小板血 (PRP) 的治疗方法在持续分娩和子宫内膜附着方面存在局限性.
- 需要改进的方法来提高子宫内膜修复的PRP疗效.
研究的目的:
- 开发和评估用于持续释放PRP的双网络 (DN) 水凝.
- 在临床环境中评估DN水凝在治疗子宫内粘附 (IUA) 的有效性.
- 阐明DN水凝对子宫内膜的再生作用的机制.
主要方法:
- 使用氨酸和交联PRP制备双网 (DN) 水凝.
- 在体外和体内评估水凝稳定性和生长因子释放 (PDGF-BB,VEGF).
- 评价子宫内皮细胞在器官中的增殖和IUA患者的临床治疗.
主要成果:
- 在PBS和体内,DN水凝表现出稳定性,PDGF-BB和VEGF持续释放.
- 在IUA患者中,DN水凝促进了子宫内皮细胞增殖,改善了临床结果,包括月经量增加和粘附度降低.
- 单细胞测序显示了WNT信号通路的丰富以及治疗后增加的SOX9和LGR5表达.
结论:
- 开发的双网络水凝有效增强了用于子宫内粘附治疗的PRP输送.
- DN水凝促进子宫内膜再生和修复,可能通过WNT通路激活.
- 这一策略为使用PRP进行子宫内膜再生提供了一个有前途的临床方法.
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