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解读香 (Musa spp.) 内部和它们之间的遗传关系使用ISSR和SRAP标记物的基因组

Roshida Soraisham¹, Punshi Tongbram¹, Surendrakumar Singh Thingnam¹

¹Department of Biotechnology, School of Life Sciences, Mizoram University, Aizawl 796004, India.

Journal, genetic engineering & biotechnology

|August 25, 2025

概括

准确的香基因组鉴定是一项挑战. 与序列相关的增强多态性 (SRAP) 标记物比简单序列重复性 (ISSR) 标记物更有效地区分Musa spp. 通过基因组加入.

科学领域:

植物遗传学
分子生物学
农业科学

背景情况:

香 (Musa spp.) 的准确表征基因组对于繁殖和保护至关重要.
传统的形态和一些分子标记在区分香加入方面存在局限性.
了解遗传关系有助于作物改良和多样性评估.

研究的目的:

评估来自5个基因组的28个香加入之间的遗传关系.
在区分香基因组中比较序列相关放大多态 (SRAP) 和简单序列重复 (ISSR) 标记的有效性.
为了确定最适合的分子标记,准确的香分类.

主要方法:

使用SRAP和ISSR标记的28个香加入的遗传多样性分析.
评估基因关系参数,包括基因多样性,多态信息含量 (PIC),总基因多样性 (Ht),种群内的基因多样性 (Hs),基因差异化系数 (Gst) 和基因流量 (Nm).
构建树状图和人口结构分析以可视化遗传关系.

主要成果:

这两种SRAP和ISSR标记都显示出与已建立的基因组相一致的遗传关系.
SRAP标记产生的平均基因多样性 (0. 69对0. 64),PIC (0. 64对0. 59),Ht (0. 39对0. 34) 和Hs (0. 19对0. 12) 的值较高.
与ISSR标志物相比,SRAP标志物在按基因组和 ploidy 级别区分香加入时表现出更高的有效性.

结论:

根据基因组对香加入的准确歧视,SRAP标记比ISSR标记更有效.
这些发现支持使用SRAP标记来准确分类和管理香遗传资源.
这项研究为种植者和研究人员提供了有价值的工具,

关键词:

香遗传关系基因组组其他国家穆萨没有SRAP

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