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在Bacillus subtilis中优化酵母的新型信号工具箱

Tomislav Vološen¹, Henri Max Deda², Anica Walther³

¹General Microbiology, Institute of Microbiology, TUD Dresden University of Technology, Dresden, Germany; Department of Aquatic Microbiology, Institute of Microbiology, University of Greifswald, Greifswald, Germany.

Journal, genetic engineering & biotechnology

|August 25, 2025

概括

开发出74个信号 (SP) 的多功能工具箱,用于增强 Bacillus subtilis 的异质蛋白质分泌. 这种方法优化了感兴趣的蛋白质 (POI) 配对,以提高生物生产产量.

科学领域:

微生物学
生物技术
分子生物学

背景情况:

有效的蛋白质分泌对于具有成本效益的生物生产至关重要.
细菌中的Sec分泌系统被广泛用于工业蛋白质生产.
预测最佳的信号 (SP) 和感兴趣的蛋白质 (POI) 的分泌是具有挑战性的.

研究的目的:

使用 Bacillus subtilis Sec 分泌系统优化异质蛋白质分泌.
为选SP-POI配对开发一种多功能工具箱方法.
确定有效的SP来增强特定酵母的分泌.

主要方法:

开发了一个整合性评估向量,以方便促进器,SP和编码序列 (CDS) 的交换.
创建了74个本地Bacillus subtilis SP的工具箱,用于与POI进行融合.
使用酵母α-和P-作为POI来测试分泌效率.
通过间接ELISA测定验证表达和分泌.

主要成果:

在Bacillus subtilis中成功表达和分泌两个酵母.
在74种SP中,有8种促进了P-费洛蒙分泌,最高水平为43nM.
三种SP有效地实现了α-费洛蒙的表达,达到8nM的最大分泌水平.

结论:

强调需要多功能选方法来确定最佳的SP-POI配对.
提供了一个强大的工具箱,易于交换的元素来改善异质蛋白质分泌.
通过优化蛋白质分泌来提高生物生产产量的实用方法.

关键词:

细菌细菌的表达方式的分泌检测系统工具箱酵母

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