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相关概念视频

MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.0K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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相关实验视频

Updated: Sep 10, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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在集群援助之外,ERH促进了初级microRNA处理

Harim Jang1,2, Junyoung Park1,2, V Narry Kim3,4

  • 1Center for RNA Research, Institute for Basic Science, Seoul, Korea.

Nature communications
|August 25, 2025
PubMed
概括
此摘要是机器生成的。

ERH和SAFB2是帮助处理初级微RNA (pri-miRNA) 的辅助因子. 这项研究显示ERH大大增强了pri-miRNA的处理,而SAFB2则特别支持聚类pri-miRNA.

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相关实验视频

Last Updated: Sep 10, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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科学领域:

  • 分子生物学
  • 核糖核酸生物学
  • 基因调控

背景情况:

  • 微RNA (miRNA) 生物发生对于基因调节至关重要.
  • 微处理器复合体 (DROSHA-DGCR8) 从初级miRNAs (pri-miRNAs) 开始进行miRNA成熟.
  • 已知ERH和SAFB2是增强微处理器功能的辅助因素,特别是在集群辅助中.

研究的目的:

  • 阐明ERH和SAFB2在primiRNA处理中的独特分子机制.
  • 了解它们在pri-miRNA集群援助中的作用.
  • 在miRNA成熟过程中提出它们的功能模型.

主要方法:

  • 使用primiRNA处理试验研究ERH和SAFB2功能.
  • 分析了ERH和SAFB2对独立和集群的primiRNA的影响.
  • 通过淘汰实验来评估ERH和SAFB2耗尽的影响.

主要成果:

  • 无论基因组背景如何 (独立或集群),ERH大大增强了primiRNA处理.
  • 与高效针相比,ERH抑制不成比例地影响了低于最佳的primiRNA针.
  • 特别地,SAFB2促进了群体内低于最佳的pri-miRNA发针的处理.

结论:

  • 在primiRNA集群中,ERH和SAFB2具有不同的作用.
  • 一个新的模型提出SAFB2可以促进微处理器在针头之间传输.
  • 建议ERH能够有效地处理非最佳的primiRNAs.