相关实验视频

Updated: May 2, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

癌症组织病变中的多参数细胞和空间组织

Mark Buckup^1,2,3, Igor Figueiredo^1,2,3, Giorgio Ioannou^1,2,3

¹Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Nature biomedical engineering

|August 25, 2025

概括

我们开发了MARQO, 一个用于多重成像分析的自动化管道, 这种工具提高了复杂蛋白质组学研究的效率和准确性,改善了癌症研究结果.

科学领域:

计算生物学
病理学
免疫组织化学

背景情况:

多重免疫染色分析是复杂的,劳动密集的和碎片化的.
目前的组织蛋白质组方法面临着越来越复杂的标志物挑战.
需要简化,自动化单细胞组织分析的解决方案.

研究的目的:

介绍MARQO,一个开源的,用于多重成像分析的自动管道.
简化整片组织的单细胞分辨率分析.
根据手工测定和现有的量化方法验证MARQO的性能.

主要方法:

MARQO集成了弹性图像注册,代核细分和无监督集群 (小型批量k-means).
一个图形界面允许用户指导细胞分类.
验证涉及人类瘤和相邻的正常组织的多重免疫组织化学染色.

主要成果:

MARQO成功地分析了各种组织大小和染色方法 (单色IHC,20色多色IF).
该管道准确地确定了各种固体癌症的标志性共表达模式.
在接受新辅助赛米利马布治疗的肝细胞癌患者中,MARQO发现了CD8+ T细胞丰富.

结论:

MARQO提供了一种高效准确的解决方案,用于单细胞分辨率的多重成像分析.
该管道适用于各种组织类型,尺寸和染色方法.
对于了解癌症生物学和治疗反应至关重要的空间解决机制.

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相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...