在雄性小鼠中减轻诱导的生殖毒性
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Published on: February 26, 2014
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Amir Mohammad Rohanii1, Tohid Mohammadi2
1Department of Basic Science, Faculty of Veterinary Medicine, Ur.C., Islamic Azad University, Urmia, Iran.
Scientific reports
|August 25, 2025
概括
复星 (RSV) 和β- 葡萄糖 (βG) 保护雄性小鼠免受 (Pb) 的生殖毒性. 综合治疗改善了精子参数和减少了氧化应激,这表明抗氧化和抗丧的益处.
科学领域:
- 毒理学
- 生殖生物学
- 营养科学
背景情况:
- (Pb) 暴露是已知的男性生殖毒素,但机制尚不清楚.
- 复星 (RSV) 和β- 葡萄糖 (βG) 具有抗氧化特性.
- 没有充分研究RSV和βG对Pb毒性的联合保护作用.
研究的目的:
- 研究白醇 (RSV) 和β-葡萄糖 (βG) 对雄性小鼠的 (Pb) 诱导的生殖毒性的协同保护作用.
- 评估RSV和βG联合治疗对精子参数,氧化应激,激素水平和丸亡的影响.
主要方法:
- 雄性小鼠接受了酸盐 (Pb) 治疗,并用RSV,βG或两者一起治疗35天.
- 评估精子数量,运动性,活力和DNA损伤.
- 评估了氧化应激标志物 (TAC,SOD,GPx,MDA),激素水平 (,LH,FSH),丸组织病理以及与亡相关的基因表达 (Bax,Bcl-2,caspase-3).
主要成果:
- 与仅服用Pb组相比,联合RSV和βG治疗显著改善了精子运动率 (61. 5%),精子数量 (87. 2%) 和减少了精子DNA损伤 (68. 1%).
- 组合疗法降低了氧化应激标志物 (MDA为63. 9%) 和增强了抗氧化能力 (TAC,SOD,GPx).
- 激素水平和抗亡的Bcl-2表达得到恢复,而亲亡的Bax和caspase-3表达则下降.
结论:
- 在临床前的小鼠模型中,resveratrol (RSV) 和β-glucan (βG) 对 (Pb) 诱导的雄性生殖毒性具有显著的协同保护作用.
- 保护机制包括强大的抗氧化活性和亡途径的调节.
- 联合RSV和βG补充剂可能是缓解Pb诱导的男性不孕症的可行策略.
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