喷或升华:三种常见正离子模式矩阵的矩阵应用考虑
Ian Spears1, Gabriella Lagdameo1, Alyson Black2
1The Johns Hopkins University Applied Imaging Mass Spectrometry Core and Service Center; Division of Cancer Imaging Research; The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine; Baltimore, Maryland, 21205, United States.
矩阵辅助激光脱离/电离质谱成像 (MALDI MSI) 矩阵沉积方法影响晶体大小和分子检测. 与喷相比,化可最大限度地减少晶体大小,但可能会降低某些脂质的信号强度.
科学领域:
- 分析化学
- 生物化学
- 质谱学
背景情况:
- 矩阵辅助激光脱离/电离质谱成像 (MALDI MSI) 能够在现场进行分子分析.
- 有效的矩阵沉积对于平衡分析物提取和最大限度地减少移位至关重要.
- 对于高分辨率的MALDI MSI来说,最小化矩阵晶体大小至关重要.
研究的目的:
- 在脂质成像中对矩阵沉积进行气动喷射和升华进行比较.
- 评估不同矩阵对晶体大小,分析物移位和信号强度的影响.
- 评估矩阵沉积方法对生物组织中的脂质检测的影响.
主要方法:
- 商用自动气动喷雾器和基质沉积器的比较
- 测试三个矩阵:CHCA,DHAP和DHB.
- 对小鼠大脑,心脏和脏组织的脂质成像分析.
主要成果:
- 基质晶体大小根据基质和沉积位置 (在组织上或外) 变化.
- 喷导致脂质的一个子集的移位增加.
- 化导致脂质子集的信号强度下降.
结论:
- 喷射和升华对MALDI MSI脂质成像具有明显的影响.
- 矩阵选择和沉积方法影响晶体大小,分析物的移位和信号检测.
- 优化矩阵沉积是高质量的MALDIMSI脂质分析的关键.
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