使用Caco-2跨井模型对低分子量高级糖化最终产品 (AGEs) 的肠道运输进行指纹检测

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Updated: Sep 10, 2025

Using Caco-2 Cells to Study Lipid Transport by the Intestine

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Published on: August 20, 2015

Xiyu Li^1,2, Sebastiaan Wesseling¹, Yaxin Sang²

¹Division of Toxicology, Wageningen University and Research, 6708 WE Wageningen, The Netherlands.

Journal of agricultural and food chemistry

|August 26, 2025

概括

食物中的高级糖化最终产品 (AGEs) 可以进入人体,吸收程度不同. 这项研究描述了10种低分子质量的AGE,揭示了肠道吸收和细胞积累的差异.

科学领域:

生物化学
营养科学
毒理学

背景情况:

食物中产生的高级糖化最终产品 (AGEs) 可能会导致内源性的AGE积累.
了解特定AGE的肠道吸收和细胞内积累对于评估其全身生物可用性至关重要.

研究的目的:

描述10个选择的低分子质量 (LMM) AGEs的肠道吸收和细胞内积累.
调查LMM AGEs系统生物可用性的潜在差异.
探索AGE结构和肠道运输之间的关系.

主要方法:

使用Caco-2跨井模型模拟肠道吸收.
在2小时内量化了10LMM AGEs的传输百分比和细胞内积累.
应用量化结构-活动关系 (QSAR) 来分析吸收率.

主要成果:

所有测试的LMM AGEs都显示出通过肠道屏障的细胞间传输有限.
甘油酸-胺 (GALA) 的运输率最高 (1.1% ± 0.3%),而N-ε- 氧甲基) 胺 (CML) 的细胞内积累率最高 (3.5% ± 0.6%).
交叉链接的AGE吸收和积累很差;被动运输可能会影响LMM AGE的吸收,受分子性质的影响.

结论:

在LMM AGEs的肠道生物可用性和细胞内积累方面存在显著差异.
这项研究提供了一种基于其分子特征的指纹检测肠道AGEs的新方法.
这些发现强调了在评估饮食中的AGE暴露和健康影响时考虑特定AGE结构的重要性.

关键词:

先进的糖化最终产品批量测试卡科2细胞穿过井的模型混合物 AGEs 数量结构与活动关系

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