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在单克隆性乳腺病患者中对氨酸转移酶的分析干扰
Sara Martínez-Rodríguez1, Alberto Izquierdo-Martínez1, Juan Miguel Guerrero Montávez1
1Clinical Analysis Laboratory, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
The journal of applied laboratory medicine
|August 26, 2025
概括
氨酸转移酶 (ALT) 测定中的分析干扰与免疫球蛋白水平升高有关,特别是单克隆性胃病变. 这一发现有助于通过常规ALT检测诊断未知的单克隆性乳腺病变.
科学领域:
- 临床化学
- 免疫学
- 实验室医学
背景情况:
- 分析干扰在临床实验室中普遍存在,因此需要强有力的检测策略来防止诊断和治疗错误.
- 必须系统地排除测试中的常见干扰,如脂血和血液溶解.
研究的目的:
- 在阿胺转移酶2 (ALT2) 试验中调查与吸收相关的单一误差 (1040).
- 在Abbott Alinity平台上7个月内观察到158个样本的干扰的根本原因.
主要方法:
- 使用Abbott Alinity c平台对在ALT2测试中表现出特定1040误差的样品进行分析.
- 已知干扰如高脂血症,血液溶解或总蛋白质度的样本被排除在外.
- 从受影响的样本中分离和定量免疫球蛋白 (Ig) 水平.
主要成果:
- 单克隆免疫球蛋白 (149 IgM,7 IgG,2 IgA) 的高度被确定为ALT测定干扰的原因.
- 与检测到的干扰相关的血清粘度增加.
- 用dithiothreitol治疗证实了IgM五分离可以消除ALT测定误差.
结论:
- ALT测试干扰显示了单克隆性胃病变的100%阳性预测值.
- 在没有先前Ig测试的患者中检测到这种特定干扰表明了以前未被识别的单克隆性胃病的潜在诊断.
- 这突显了常规ALT测试在潜在的未诊断的单克隆性乳腺病变的有用性.
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