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在红耳滑鼠 (Trachemys scripta elegans) 的温度依赖性性别确定和分化过程中,通过RNA-seq进行淋巴体转录组分析

Jiawei Fan¹, Fang Li², Lingyuan Lou³

¹College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|August 26, 2025

概括

在海中取决于温度的性别确定 (TSD) 是复杂的. 这项研究揭示了关键的基因和替代拼接,

科学领域:

* 发育生物学
* 基因组学
* 分子内分泌学

背景情况:

在许多爬行动物中,温度依赖性性别判定 (TSD) 对于性别差异化至关重要.
* 调节TSD的分子途径在很大程度上是未知的.
了解TSD机制对于预测物种对气候变化的反应至关重要.

研究的目的:

* 在红耳 (*Trachemys scripta elegans*) 中确定温度依赖性性别的分子机制.
* 在雄性和雌性生产温度下研究胚胎性阴茎的基因表达和替代拼接模式.

主要方法:

* 在雄性生产温度 (MPT) 和雌性生产温度 (FPT) 中化胚胎海性腺体的转录组序列.
* 差异基因表达分析以确定特定阶段的基因候选.
* 基因共同表达网络分析以发现潜在的枢纽基因和调节途径.

主要成果:

* 通过MPT或FPT确定了许多差异表达的基因和关键候选基因.
* DHRS11被确定为MPT中的潜在热敏枢纽基因;ATP2B4 (转运器) 作为FPT中的枢纽基因,这表明信号的作用.
* 发现了广泛的替代拼接,包括在JARID2和KDM6B中保持内子,可能受到CIRBP的监管.

结论:

* 转录和转录后调节,包括信号和温度响应拼接,是爬行动物TSD的关键机制.
这些发现为TSD的分子基础及其在适应环境温度变化的潜在作用提供了新的见解.

关键词:

替代拼接没有.一个 TSD 转录组

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