狐基因在钻石背的发育和繁殖中的作用
Anam Noreen Abbas1, Mubashir Tariq1, Faisal Munir1
1State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China; Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China; Key Laboratory of Green Pest Control, Fujian Province University, Fuzhou 350002, China.
叉头盒1 (PxFox1) 基因对钻石背的发展和繁殖至关重要. 在Plutella xylostella中淘汰PxFox1显著损害了卵子的生产,生存,并导致发育异常,这表明它是害虫控制的目标.
科学领域:
- 昆虫分子生物学
- 发育遗传学
- 农业昆虫学
背景情况:
- 叉头盒 (狐) 转录因子调节昆虫的重要功能.
- 狐基因在钻石背 (Plutella xylostella) 中的作用,这是一个关键的十字虫害,仍然没有特征.
- 了解PxFox1对于管理P. xylostella至关重要.
研究的目的:
- 在P. xylostella中描述PxFox1基因.
- 研究PxFox1在昆虫繁殖和发育中的作用.
- 探索PxFox1作为一种遗传害虫控制的目标.
主要方法:
- 在P. xylostella中识别和基因组分析PxFox1.
- 对不同发育阶段和组织的基因表达分析.
- 通过CRISPR/Cas9基因编辑创造出PxFox1的淘汰突变.
- 突变的表型分析,包括生殖输出,发育时间和形态.
主要成果:
- PxFox1在类动物中保存,在成年雌性和体中高度表达.
- PxFox1淘汰突变的卵数量和化率显著降低.
- 突变者表现出延长幼虫寿命,缩短幼儿期,缩短成人的寿命,以及严重的形态缺陷 (例如,形的翅膀,不规则的细分,体型较小).
结论:
- 对于P. xylostella的正常发育,生存和繁殖,PxFox1是不可或缺的.
- 针对PxFox1是一个有前途的策略,用于开发新的遗传害虫控制方法.
- 这项研究提供了关于昆虫基因调控的见解,
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