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Updated: Sep 10, 2025

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Circadian Entrainment of Drosophila Melanogaster
Published on: June 3, 2020
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夜间人工光对虫的行为节律的性二态和时钟基因特异性影响
Ausra Pranevicius1, Grace Biondi1, Aishwarya Ramakrishnan Iyer2
1Neuroscience and Behavior, Barnard College, New York, NY 10027, USA.
Proceedings. Biological sciences
|August 26, 2025
概括
人工夜间照明 (ALAN) 破坏昼夜节律. 不同于循环基因,Drosophila基因周期的丧失会使ALAN诱导的夜间行为恶化,特别是在雄性中.
科学领域:
- 时间生物学
- 环境科学
- 遗传学
背景情况:
- 光污染是影响生态系统的重大环境变化.
- 人工夜间照明 (ALAN) 破坏了各种物种的昼夜节律.
- 对ALAN影响的神经和遗传机制尚不清楚.
研究的目的:
- 研究生物钟基因对Drosophila对ALAN的反应中的作用.
- 确定月经和周期基因的突变如何影响ALAN下的行为.
- 阐明调节行为可塑性对环境变化的遗传因素.
主要方法:
- 使用Drosophila melanogaster作为一个模型生物.
- 研究ALAN对野生类型和突变的影响 (周期和周期淘汰).
- 使用CRISPR-Cas9在时钟神经元中产生向期基因淘汰.
主要成果:
- 月经基因的丧失加剧了ALAN诱导的夜间行为,特别是在男性中.
- 循环基因的丧失没有显著改变对ALAN的反应.
- 在时钟神经元中,CRISPR-Cas9的淘汰效应模仿了零突变的表型.
- 在阳性和阴性时钟基因肢体的突变之间观察到不同的ALAN反应.
结论:
- 遗传因素,特别是生物钟基因的突变, 显著调节对光污染的行为反应.
- 周期和周期基因在介导ALAN对昼夜行为的影响方面发挥着差异性作用.
- 这些发现突显了在面对环境变化时对行为可塑性的遗传影响的重要性.
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