在外热脊椎动物中肌肉OXPHOS效率的进化多样性
Rachel Lockridge Mueller1, Lance C Li Puma2, Michael W Itgen1
1Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
Proceedings. Biological sciences
|August 26, 2025
概括
内热生物在线粒体效率上表现出显著的差异,内膜质子透率在物种之间产生能量和反应性氧物种的差异. 这些发现突显了氧化代谢中的多种进化适应.
科学领域:
- 比较生理学
- 线粒体生物能学
- 进化生物学
背景情况:
- 氧化化 (OXPHOS) 对于脊椎动物的能量产生至关重要.
- OXPHOS合效率会影响热生成,运动和内热体中的反应性氧物种 (ROS).
- 对OXPHOS合的变化和影响仍然不太清楚.
研究的目的:
- 为了研究线粒体OXPHOS合效率在ectotherm物种的变化.
- 确定标准代谢率 (SMR) 和线粒体功能之间的关系.
- 确定OXPHOS合的基本机制.
主要方法:
- 测量了线粒体的氧气消耗,ATP的产生和ROS的产生.
- 使用了,青和的透气化骨肌肉纤维.
- 评估了内在质子透性和特定的质子泄漏途径.
主要成果:
- 的ATP产能最高,而的产能最低,与SMR相关.
- 氧气消耗率出现了意想不到的反向趋势,显示了OXPHOS合效率的8.5倍变化.
- 内线粒体膜的内在质子透性是这些变异的主要驱动因素,在中最高,在中最低.
- 通过解蛋白和腺因核酸转位酶表现出基底质子泄漏,限制了ROS的产生.
结论:
- 肌肉OXPHOS效率存在显著的进化变异.
- 内在质子透性是调节OXPHOS合和ROS产生的一个关键因素.
- 多种选择性压力塑造了线粒体功能及其生理后果.
相关概念视频
Energy Supply for Muscle Contraction
3.7K
Skeletal muscle fibers have the unique ability to switch between rest and contraction states, using different sources of ATP for energy. The contraction cycle and Ca2+ transport back into the sarcoplasmic reticulum for relaxation require significant ATP. However, the ATP reserves in muscle fibers are limited and can only sustain contractions for a few seconds. Additional ATP production becomes necessary for prolonged contractions. As a result, muscle fibers generate ATP through various sources,...
3.7K
Production Efficiency
17.0K
Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
17.0K
Peroxisomes and Mitochondria
88.7K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
88.7K
Animal Mitochondrial Genetics
8.0K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
8.0K


