为可扩展和高效的基于RNAi的农药设计的RNA纳米结构
Ying Xia1, Tao Zhang1, Xiaokun Ni1
1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, PR China.
Trends in biotechnology
|August 26, 2025
概括
自组装RNA纳米结构 (SARNs) 为RNA干扰 (RNAi) 农药提供了稳定,可扩展的解决方案. 在农业和生物医学中提升基因沉默应用.
科学领域:
- 农业科学
- 生物技术
- 分子生物学
背景情况:
- 双链RNA (dsRNA) 农药在稳定性,可扩展性和传递性方面存在局限性.
- 有效的RNA干扰 (RNAi) 需要农业和生物医学应用的强大的传递系统.
研究的目的:
- 设计自组装RNA纳米结构 (SARNs) 以提高RNAi杀虫剂的性能.
- 在植物和昆虫模型中评估SARN的稳定性,可扩展性和有效性.
- 建立一个从实验室到现场应用的SARN过渡框架.
主要方法:
- 带有功能性小干扰RNA (siRNA) 的自组装RNA纳米结构.
- 嵌入的基因增强siRNA的疏水性和弹性,以改善释放动力学.
- 在不同的食习惯的植物和昆虫模型中测试了SARN (Tribolium castaneum和Nilaparvata lugens).
- 与传统的dsRNA相比,评估了RNAi的效率,基因下调和死亡率.
主要成果:
- 在植物和昆虫害虫中,SARNs表现出较好的RNA稳定性和传递性.
- 与dRNA相比,SARN在虫和穿孔吸虫虫害中实现了更高的RNAi效率,基因下调和死亡率.
- 通过大肠杆菌的转录,SARNs可以进行可扩展的生成.
结论:
- 基于RNA的基因沉默是一种高效,可扩展和低成本的平台.
- 这种工程RNA技术促进了RNAi在农业和生物医学中的应用.
- 解决与dRNA农药开发和部署相关的关键挑战.
相关概念视频
02:15Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
01:23RNA Interference
26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K
02:30siRNA - Small Interfering RNAs
17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
02:57piRNA - Piwi-interacting RNAs
7.0K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.0K
02:30Small interfering RNAs (siRNA)
3.6K
3.6K