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细菌微生物组在周围植入物条件下进行分析

Joaquin Espinoza-Arrue¹, Marion Arce^1,2,3, Natalia Endo¹

¹Laboratory of Oral Microbiology and Immunology, Faculty of Dentistry, University of Chile, Santiago, Chile.

Journal of clinical periodontology

|August 26, 2025

概括

周植入物微生物组在健康,粘膜炎和周植入物疾病之间存在显著差异. 周植入炎显示出细菌多样性增加和疾病,由特定的致病物种驱动.

科学领域:

口腔微生物学
牙周病学
微生物组研究

背景情况:

细菌微生物组在维持植入周健康方面发挥着至关重要的作用.
周植入物微生物组的变化与周植入物粘膜炎和周植入物炎等炎症状况有关.

研究的目的:

在植入周健康,植入周粘膜炎和植入周炎中全面描述细菌微生物组.
确定与这些疾病相关的关键细菌物种和功能途径.

主要方法:

从15项研究 (522个样本) 中重新分析了16S rRNA测序数据.
使用Mothur进行预处理,使用HOMD数据库进行分类.
分析多样性,相对丰富性,差异性特征 (LEfSe),功能潜力 (PICRUSt2),并发网络和失能指数.

主要成果:

与健康和围膜炎相比,围膜炎的细菌多样性和丰富性更高.
对于每种情况,观察到不同的社区结构和共同发生网络.
发现了主要物种:Rothia spp. 和 Veillonella parvula_dispar (健康状况); Leptotrichia spp. 和Fusobacterium核亚种. 多形体 (粘膜炎); Porphyromonas gingivalis,F.核亚种维森蒂菌和坦内瑞拉菌 (植入周炎).
周植入炎显示预测的致病途径和更大的细菌失调.

结论:

周围植入物微生物组合和功能在健康,粘膜炎和周围植入物炎之间有显著差异.
特定的细菌物种和不良微生物群体与周植入炎有很强的关联.
这些发现提供了关于围部植入物炎症和破坏的微生物驱动因素的见解.

关键词:

16S 核糖体RNA 牙科植入物高通量核酸测序微生物群周围植入物炎

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