基于生物信息学和机器学习模型的枢纽基因和肌损伤相关的免疫细胞的查
Shulong Sun1, Hua Li1, Yan Li1
1Department of Emergency Trauma Center, Henan University of Chinese Medicine Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou, China.
Scientific reports
|August 26, 2025
概括
这项研究揭示了MYH1和MYH6等关键基因参与肌愈合机制. 巨M2细胞被认为是肌修复炎症阶段的关键参与者.
科学领域:
- 生物医学工程
- 分子生物学
- 基因组学
背景情况:
- 肌损伤由于复杂的愈合过程而带来重大临床挑战.
- 了解肌愈合的分子机制对于开发有效治疗至关重要.
研究的目的:
- 阐明肌愈合的分子机制.
- 为临床应用确定修复中的关键基因和途径.
主要方法:
- 使用来自GEO数据库的GSE26051数据集和R语言进行差异基因表达分析.
- 综合基因表达数据与来自GeneCards的肌损伤基因组,使用STRING和Cytoscape构建蛋白质-蛋白质相互作用网络.
- 应用机器学习模型用于中心基因识别,并进行基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
主要成果:
- 鉴定了721个与肌损伤相关的差异表达基因 (DEG) 和228个常见基因.
- 突出显示MYH6,MYL3,MYH1,MYH8,MYL1,TTN,TCAP,PKP2,ACTN2和CSRP3作为顶级候选基因,MYH1,MYH6,PKP2和MYH8被确定为关键的枢纽基因.
- 发现肌肉细胞细胞骨通路中枢基因的丰富,并确定M2巨在肌愈合的炎症阶段具有重要作用.
结论:
- MYH1,MYH6,MYH8和PKP2是肌愈合的关键基因,主要与肌肉细胞细胞骨通路有关.
- 巨M2在肌愈合的炎症阶段起着至关重要的作用,提供潜在的治疗点.
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