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研究领域工程学纳米技术分子和有机电子
用于检测过氧化和酸的铁素功能化氧群纳米酶

用于检测过氧化和酸的铁素功能化氧群纳米酶

Shijie Wang ¹, Yan Cheng ², Zhelin Liu ¹, Xiangting Dong ¹, Bo Zhao ³

Shijie Wang ¹, Yan Cheng ², Zhelin Liu ¹

¹School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, P. R. China.
²Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, College of Life Science, Jilin Agricultural University, Changchun, Jilin, 130118, P. R. China.
³School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, P. R. China. bozhao@cust.edu.cn.

⁰School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, P. R. China.

Analytical and Bioanalytical Chemistry +

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2025年八月26日

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在PubMed上查看摘要

概括

此摘要是机器生成的。

这项研究引入了一种新的铁素功能化氧集群,[In7Fc6],表现出类似过氧化酶的活性. 这种纳米酶能够在生物样本中灵敏地测定过氧化 (H2O2) 和酸 (AA).

科学领域

* 纳米材料科学
* 催化剂
* 生物医学感应

背景情况

*纳米酶比天然酶具有优势,包括提高稳定性和降低成本.
* 在催化和传感应用中探索铁素功能化纳米材料.
* 氧集群是一种具有潜在酶类活性的新型纳米材料.

研究的目的

* 为了研究一种新的铁素功能化氧集群的类似酶的活性, [In7Fc6].
* 开发使用[In7Fc6]集群检测过氧化 (H2O2) 的色度传感器.
* 建立用于检测 Askorbic 酸 (AA) 的色度传感器,并验证其在生物样本中的性能.

主要方法

* 铁素功能化氧集群的合成和表征 ([In7Fc6]).
在H2O2的存在下评估[In7Fc6]与TMB,OPD和ABTS等基质的过氧酶类活性.
* 开发和优化用于检测H2O2和AA的色度传感器.
在细胞和血清等生物矩阵中验证传感器性能.

主要成果

* [In7Fc6] 集群表现出显著的过氧酶类活性,催化了TMB,OPD和ABTS被H2O2氧化.
* 开发了一种H2O2的色度传感器,其检测范围为1-170μM,检测极限为0.24μM.
* 建立了AA的色度传感器,检测3~130μM的度,检测极限为0.81μM.
* 开发的传感器在细胞和血清样本中检测H2O2和AA的实用性.

结论

* 铁素功能化氧集群[In7Fc6] 显示出有前途的过氧酶类纳米酶活性.
这种纳米酶可促进对H2O2和AA的敏感和实用色度传感器的开发.
* 这些发现突显了氧集群在先进的生物分子检测应用中的潜力.

关键词:

亚酸的使用过氧化氧集群纳米酶

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