相关实验视频
Updated: May 5, 2026

09:12
Aortic Ring Assay
Published on: November 24, 2009
31.4K
无血清大动脉环测试
Alfred C Aplin1, Giovanni Ligresti2, Roberto F Nicosia3
1Seattle Institute for Biomedical and Clinical Research, and Veterans Administration Puget Sound Health Care System, Seattle, WA, USA.
Methods in molecular biology (Clifton, N.J.)
|August 26, 2025
概括
这项研究介绍了一种小型化无血清方法,用于培养小鼠大动脉环,以研究血管生成. 这种优化的测试可使皮质细胞覆盖的内皮血管自发发芽,这对于血管生成因子的研究至关重要.
科学领域:
- 血管生物学
- 细胞培养技术
- 血管新生研究
背景情况:
- 无血清培养通过最小化纤维细胞外生增长来增强血管新血管的可视化.
- 由于大小小小的老鼠大动脉环,标准的无血清方法对大动脉环无效.
- 无血清介质中的内源因子可以刺激血管生成,但需要对小鼠模型进行优化.
研究的目的:
- 开发和验证小规模的无血清培养方法用于小鼠大动脉环.
- 在没有外源生长因子的情况下,从小鼠大动脉扩张物中实现自发血管生长.
- 促进对转基因小鼠模型中的血管性调节剂和反应的研究.
主要方法:
- 通过减少培养基体体积来缩小无血清大动脉环测定.
- 嵌入小鼠大动脉环在薄原凝中.
- 与大鼠试验相比,在无血清介质中显著减少 (1/10).
主要成果:
- 使用微型无血清方法培养的老鼠大动脉环自发地呈现出血管发芽.
- 这些芽的特征是皮质细胞覆盖的内皮血管.
- 测试显示对外源性血管性因素的反应,证实了它的有用性.
结论:
- 微型无血清大动脉环测定对于研究血管生成是有效的.
- 这种方法克服了先前技术的局限性,使自发发芽和血管调节剂的评估成为可能.
- 它为研究转基因小鼠的血管生物学提供了宝贵的工具.
相关概念视频
Blood Flow
57.5K
Blood is pumped by the heart into the aorta, the largest artery in the body, and then into increasingly smaller arteries, arterioles, and capillaries. The velocity of blood flow decreases with increased cross-sectional blood vessel area. As blood returns to the heart through venules and veins, its velocity increases. The movement of blood is encouraged by smooth muscle in the vessel walls, the movement of skeletal muscle surrounding the vessels, and one-way valves that prevent backflow.
57.5K
Assessment of blood pressure in brachial artery(one-step method)
1.4K
This procedural guide systematically measures blood pressure using an oscillometric digital sphygmomanometer, emphasizing accuracy, patient safety, and comfort.
Prepare for the Procedure:
Prepare for the Procedure:
1.4K
Assessment of blood pressure in brachial artery(two-step method)
2.1K
Measuring blood pressure is a fundamental skill in healthcare that aids in diagnosing and monitoring hypertension and other cardiovascular conditions. An aneroid sphygmomanometer, commonly used in clinical settings, offers a manual and precise method for blood pressure measurement. The technique for using this instrument involves specific steps that must be carefully executed to ensure accuracy. The following detailed description outlines a two-step technique for assessing blood pressure using...
2.1K
Assessing Blood pressure using a doppler ultrasound
3.0K
To obtain accurate blood pressure measurements in clinical settings, especially when traditional methods are insufficient, healthcare professionals utilize the Doppler ultrasound technique. This method uses high-frequency sound waves to detect blood flow within the arteries, which is crucial for patients with conditions that complicate circulatory system assessment.
Pre-Procedural Guidelines for Doppler Ultrasound Blood Pressure Assessment:
Preparation of Equipment:
Pre-Procedural Guidelines for Doppler Ultrasound Blood Pressure Assessment:
Preparation of Equipment:
3.0K
Assessment of the Cardiovascular System III: Palpation
1.6K
Palpation involves feeling the body to evaluate texture, size, consistency, and tenderness for assessing cardiovascular health. The following steps are organized in a head-to-toe order:
Jugular Venous Pressure (JVP) Measurement
Position the patient at a thirty- to forty-five-degree angle or in a semi-fowler's position. Look for the highest point of pulsation in the internal jugular vein and measure the vertical distance to the angle of Loius or sternal angle. A normal JVP is 3-4 cm above...
Jugular Venous Pressure (JVP) Measurement
Position the patient at a thirty- to forty-five-degree angle or in a semi-fowler's position. Look for the highest point of pulsation in the internal jugular vein and measure the vertical distance to the angle of Loius or sternal angle. A normal JVP is 3-4 cm above...
1.6K
Peripheral Arterial Disease II: Clinical Manifestations and Diagnostic Evaluation
773
Clinical manifestationsPeripheral Arterial Disease (PAD) manifests through a range of symptoms, from the characteristic intermittent claudication to atypical presentations and severe complications in advanced stages. Intermittent claudication, a hallmark symptom of PAD, presents as exercise-induced muscle pain that typically resolves within minutes of rest. This pain is reproducible and stems from inadequate blood flow, leading to the accumulation of lactic acid produced during anaerobic...
773

