Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Prospective Head-to-Head Comparison of HER2-Targeted and 18F-FDG PET/CT for Detecting Axillary Lymph Node Metastases Among Newly Diagnosed HER2-Positive and HER2-Low Breast Cancer.

Clinical nuclear medicine·2026
Same author

NAD<sup>+</sup> Metabolism Licenses Zygotic Genome Activation via PARP7-Mediated ADP-Ribosylation of UHRF1 in Mouse Early Embryos.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Multidimensional MRI radiomics-based model predicts recurrence risk in triple-negative breast cancer.

NPJ precision oncology·2026
Same author

Identification and Validation of ALOX15 as a Robust Diagnostic Biomarker for Obesity-Associated Asthma: An Integrative Bioinformatic and Experimental Study.

Journal of inflammation research·2026
Same author

Corrigendum to "Comprehensive Visualization of Choroidal Microvessels and Nerve Structure in Graves' Disease without Clinical Ophthalmopathy Using Ultra-Widefield OCT Angiography" [PDPDT 2026 Apr 2;59:105453].

Photodiagnosis and photodynamic therapy·2026
Same author

Discovery of ferroptosis drugs targeting SLC7A11/BECN1 based on living cell quantitative FRET imaging.

Biochimie·2026

相关实验视频

Updated: Sep 10, 2025

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

10.0K

用于定量光成像的非均照明校正

Yue Wang1,2, Yongqiang Liu1,2, Beini Sun1,2

  • 1Key Laboratory of Laser Life Science, Ministry of Education, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong, China.

Microscopy research and technique
|August 27, 2025
PubMed
概括

系统的均光学图像校正 (SUFIC) 通过纠正不均的照明来改善定量显微镜. 这种高效的前性方法需要最小的参考图像,提高光共振能量转移 (FRET) 图像的准确性.

关键词:
光成像照明的调整照明的一致性定量的FRET

更多相关视频

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

相关实验视频

Last Updated: Sep 10, 2025

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

10.0K
Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

科学领域:

  • 显微镜和成像技术
  • 生物物理
  • 定量光成像

背景情况:

  • 在光显微镜中,照明均对于准确的定量分析至关重要.
  • 现有的回顾性校正方法在有限的图像数据上扎,而前性方法则需要频繁的重新校准.
  • 不均的照明对量化光共振能量转移 (FRET) 测量有显著影响.

研究的目的:

  • 从理论和实验上分析非均照明对定量FRET的影响.
  • 引入系统统一光图像校正 (SUFIC),一种新的前照明校正方法.
  • 与现有方法相比,评估SUFIC的疗效.

主要方法:

  • 理论分析照明不均对FRET的影响.
  • 系统统一光图像校正 (SUFIC) 方法的开发和应用.
  • 使用光微球,Argo-HM幻灯片和表达FRET结构的活细胞进行实验验证.

主要成果:

  • 在Argo-HM幻灯片图像中,SUFIC显著改善了场均性 (27. 45%至65. 30%).
  • 在FRET图像中,SUFIC提高了信号与背景的比率12.3%,7.9%和20.9%.
  • SUFIC需要至少三个参考图像,比CIDRE和BaSiC方法少得多.

结论:

  • SUFIC是一种高效的前性方法,用于定量显微镜的照明校正.
  • 该方法提供了提高精度和减少光成像的重新校准需求.
  • 在各种生物样本和FRET研究中,SUFIC具有广泛的适用性.