通过提升能量迁移进行增强的单粒子升级成像
Yanxin Zhang1, Rongrong Wen1, Tianli Zhai1
1Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 27, 2025
概括
研究人员增强了化增强转化纳米粒子 (UCNPs) 以获得更明亮的生物成像. 优化UCNP可长期跟踪神经元传输,揭示了素-氨酸协调机制.
科学领域:
- 材料科学
- 纳米技术
- 神经科学
- 生物物理
背景情况:
- 用兰化物添加的上转化纳米粒子 (UCNPs) 为生物成像提供光稳定性和低背景.
- 单颗粒的有限亮度限制了它们在生物研究中的广泛应用.
- 提高能源迁移 (EM) 和转移效率对于更明亮的UCNP至关重要.
研究的目的:
- 克服UCNP的有限亮度,以改善生物成像应用.
- 增强Yb3+传感器和Er3+发射器之间的能量迁移.
- 为神经元传输研究开发具有优异单粒子亮度的UCNP.
主要方法:
- 设计的核心--UCNP (NaLu0.9Er0.1F4@NaYbF4@NaLuF4) 来抑制反向能量传输 (BET).
- 增加了核心中的Yb3+兴奋剂 (NaLu0.9-xYbxEr0.1F4@NaYbF4@NaLuF4) 来促进EM.
- 使用贝叶斯隐藏马尔科夫模型对神经元运输动态进行定量分析.
主要成果:
- 具有合金核心 (NaYb0.9Er0.1F4) 的UCNP显示上转光度增加了十倍以上.
- 优化的Yb3+/Er3+比和惰性外厚度最大化了单颗粒的亮度.
- 能够长期追踪轴突运输在背部根系神经元 (DRG) 中.
结论:
- 优化的UCNP通过改善能量迁移显著提高单颗粒的亮度.
- 揭示了神经元运输中的素-丁氨酸协调机制.
- 作为实时神经元活动监测的强大工具, 建立了单颗粒上升转换跟踪 (uSPT).
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