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相关概念视频

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
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相关实验视频

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通过提升能量迁移进行增强的单粒子升级成像

Yanxin Zhang1, Rongrong Wen1, Tianli Zhai1

  • 1Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 27, 2025
PubMed
概括

研究人员增强了化增强转化纳米粒子 (UCNPs) 以获得更明亮的生物成像. 优化UCNP可长期跟踪神经元传输,揭示了素-氨酸协调机制.

关键词:
轴突运输能源迁移一个粒子成像上升转换的发光

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科学领域:

  • 材料科学
  • 纳米技术
  • 神经科学
  • 生物物理

背景情况:

  • 用兰化物添加的上转化纳米粒子 (UCNPs) 为生物成像提供光稳定性和低背景.
  • 单颗粒的有限亮度限制了它们在生物研究中的广泛应用.
  • 提高能源迁移 (EM) 和转移效率对于更明亮的UCNP至关重要.

研究的目的:

  • 克服UCNP的有限亮度,以改善生物成像应用.
  • 增强Yb3+传感器和Er3+发射器之间的能量迁移.
  • 为神经元传输研究开发具有优异单粒子亮度的UCNP.

主要方法:

  • 设计的核心--UCNP (NaLu0.9Er0.1F4@NaYbF4@NaLuF4) 来抑制反向能量传输 (BET).
  • 增加了核心中的Yb3+兴奋剂 (NaLu0.9-xYbxEr0.1F4@NaYbF4@NaLuF4) 来促进EM.
  • 使用贝叶斯隐藏马尔科夫模型对神经元运输动态进行定量分析.

主要成果:

  • 具有合金核心 (NaYb0.9Er0.1F4) 的UCNP显示上转光度增加了十倍以上.
  • 优化的Yb3+/Er3+比和惰性外厚度最大化了单颗粒的亮度.
  • 能够长期追踪轴突运输在背部根系神经元 (DRG) 中.

结论:

  • 优化的UCNP通过改善能量迁移显著提高单颗粒的亮度.
  • 揭示了神经元运输中的素-丁氨酸协调机制.
  • 作为实时神经元活动监测的强大工具, 建立了单颗粒上升转换跟踪 (uSPT).